ICAM-1和VCAM-1单抗影响活化淋巴细胞粘附HUVEC的机理研究  

THE EFFECTS OF ICAM 1 AND VCAM 1 ON THE ADHERENCE OF ACTIVATED LYMPHOCYTES TO HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS

作　　者：韩建奎[1] 侯桂华[1] 张庆殷[1] 

机构地区：[1]山东医科大学核医学教研室

出　　处：《山东医科大学学报》2000年第3期247-249,共3页Acta Academiae Medicinae Shandong

摘　　要：目的 :研究细胞粘着分子 1(ICAM 1)和血管细胞粘着分子 1(VCAM 1)单抗对rIL 2活化的脐血淋巴细胞粘附人脐静脉内皮细胞 (HUVEC)的机理。方法 :①利用 2 4孔板培养HUVEC ,在相应孔中分别加入ICAM 1和VCAM 1单抗 ,30min后测定活化的脐血淋巴细胞和未活化的淋巴细胞粘附百分率 ;②采用桥联酶标法 (APAAP)测定粘附细胞的表型。结果 :①ICAM 1和VCAM 1单抗 (2 0mg/L)均可明显降低rIL 2活化的淋巴细胞的粘附 ,前者使粘附率由 56 54%降至 4 1 6 0 % (P <0 .0 5) ;后者使粘附率降至 30 30 % (P <0 .0 5) ;②粘附细胞的表型分析结果表明 ,ICAM 1单抗可降低CD8+ 细胞的粘附 ,增加CD4 + 细胞的粘附 ,对CD56 + 细胞的粘附无明显影响 ,而VCAM 1单抗则增加CD8+ 细胞的粘附 ,降低CD56 + 细胞的粘附 ,对CD4 +细胞无明显影响。结论 :粘着分子ICAM 1和VCAM 1参与活化脐血淋巴细胞粘附HUVEC的调控 ,其抗体虽可明显降低活化脐血淋巴细胞对HUVEC的粘附 。Objective:To explore the effects of ICAM 1 and VCAM 1 on the adherence of rIL 2 activated umbilical lymphocytes to human umbilical vein endothelial cells(HUVEC). Methods:① Activated umbilical lymphocytes were put on the surface of the cultured HUVEC sheet.After a certain period of time, the adherence rate was calculated. ② The phenotypes of adherent lymphocytes were studied with APAAP method. Results:①ICAM 1 and VCAM 1 McAb obviously lower the adherent activity of activated lymphocytes.The former decreased the adherent rate from 56.54% to 41.6%(P<0.05),and the latter to 30.3%(P<0.05). ② ICAM 1 McAb lowered the adherence of CD8 +,raised the adherence of CD4 +,and have no significant effect on CD56 +,while VCAM 1 McAb increased the adherence of CD8 +,lowered the adherence of CD56 + and have no significant effect on CD4 +. Conclusion:The adherent activity of activated lymphocytes is stronger obviously than that of unactivated ones.ICAM 1 and VCAM 1 may participate in the regulation of the adherence of activated lymphocytes to HUVEC.

关 键 词：细胞粘着分子 单克隆抗体 ICAM-1 VCAM-1 HUVEC 

分 类 号：R392.11[医药卫生—免疫学]