实验性哮喘小鼠脾细胞培养上清液IL-5的测定及其意义  

Determinations of IL-5 Contents in the Supernatants of Spleen Cell Cultures of Experimental Asthmatic Mice and its Significance

作　　者：王美琴[1] 钮善福[2] 白春学 方晓惠 陈常庆[3] 陈波[3] 

机构地区：[1]复旦大学医学院附属华山医院呼吸内科,200040 [2]复旦大学医学院附属中山医院呼吸病研究所,上海200032 [3]中国科学院上海生物工程中心

出　　处：《上海免疫学杂志》2000年第5期301-303,共3页Shanghai Journal of Immunology

摘　　要：本文欲探讨IL 5在哮喘发病机制中的作用。用卵蛋白 (OVA)造模形成哮喘鼠 ,取其脾T淋巴细胞体外培养。以ELISA法测定细胞培养上清液中IL 5浓度。结果经OVA刺激的哮喘鼠T淋巴细胞培养上清液中IL 5 (4 4 6± 6 2pg/ml)明显增高 ;与健康鼠比较有显著性差别 (P <0 0 1)。认为T淋巴细胞产生的IL 5与哮喘气道炎症可能有关。To study the role of IL-5 in the pathogenesis of asthma,the T lymphocytes in spleen cells of asthmatic mouse model induced by ovalbumin(OVA) were cultivated in vitro,and the IL-5 contents in the cell culture supernatants were determined by ELISA It was found that IL-5 was markedly increased in the supernatants of spleen cell cultures of mice sensitized with OVA (44 6±6 2pg/ml) with significant differences between the control healthy mice It concludes that the IL-5 produced by T lymphocytes might be related with the airway inflammations in asthma

关 键 词：哮喘 小鼠 IL-5 脾细胞培养上清液 测定 

分 类 号：R562.25[医药卫生—呼吸系统]