利湿合剂的定性及定量  

Qualitative and Quantitative Analysis for Lishi Mixture

作　　者：李近磊[1] 蒋秀娟 付文焕[1] 钟明康[1] 

机构地区：[1]复旦大学附属华山医院,上海200040 [2]青州市人民医院,山东青州262500

出　　处：《中国医药工业杂志》2013年第5期492-495,共4页Chinese Journal of Pharmaceuticals

摘　　要：建立了利湿合剂的药材质控方法。以高效液相色谱法测定其中的苦参碱。采用Diamonsil C18色谱柱,甲醇-水-25%氨水(52:45:3)为流动相,检测波长215 nm。苦参碱在0.64～3.2 g范围内线性关系良好,回收率为95.6%,RSD为2.85%。以薄层色谱法鉴别苦参和陈皮,用高效液相色谱法鉴别金银花中的绿原酸。The methods of the medicinal quality control for Lishi mixture was established. The matrine in Lishi mixture was determined by HPLC. A Diamonsil C^s column was used with the mobile phase of methanol-water-25 % ammonium hydroxide （52 ： 45 ： 3） at the detection wavelength of 215 nm. The calibration curve was linear in the range of 0.64 - 3.2 ~tg. Its recovery was 95.6%, with RSD of 2.85 %. Radix Sophoraflavescentis and Pericarpium citri reticulatae were identified by TLC. Chlorogenic acid in Flos loniceraejaponicae was identified by HPLC.

关 键 词：苦参 陈皮 金银花 绿原酸 高效液相色谱 薄层色谱 鉴别 测定 

分 类 号：O657.72[理学—分析化学] TQ460.71[理学—化学]