可降解胸骨固定材料聚丁二酸丁二醇酯对THP-1细胞体外生物学特性的影响  

Effects of a biodegradable sternal fixation material poly(butylene succinate)on the biological characteristics of THP-1 cells in vitro

作　　者：段长恩[1] 公兵[1] 杨秀滨[1] 

机构地区：[1]北京协和医学院,中国医学科学院,阜外心血管病医院心脏外科,100037

出　　处：《实用医学杂志》2013年第9期1391-1394,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:30870620);北京自然科学基金资助项目(编号:2112045)

摘　　要：目的:研究生物可降解胸骨固定材料聚丁二酸丁二醇酯(PBS)对THP-1细胞体外生物学特性的影响。方法:EdU和Hoechst 33342染色法分别研究PBS浸提液共培养后THP-1细胞的增殖和凋亡;RT-qPCR检测细胞凋亡相关基因Bax/Bcl-2和Smac/IAP-1的表达变化;染色体畸变试验评价其基因毒性。结果:PBS浸提液对THP-1细胞的体外增殖率影响较小,不存在细胞增殖半数抑制剂量;浸提液共培养后的细胞出现部分凋亡,基因表达改变以Bax/Bcl-2显著(P<0.05),Smac/IAP-1变化不明显(P>0.05);最大浓度浸提液共培养后的细胞染色体畸变率在正常范围。结论:PBS对THP-1细胞的体外生物学特性影响较小,对该细胞显示良好的生物相容性。Objective To evaluate the effects of PBS on the biological characteristics of THP-1 cells in vitro.Methods The EdU and Hoechst 33342 staining were used to assess the proliferation and apoptosis of THP-1 cells co-cultured with the extracts of the materials respectively.The expression of Bax /Bcl-2 and Smac /IAP-1 related to apoptosis were evaluated by RT-qPCR.The genotoxicity of extract was evaluated by chromosomal aberration test.Results The extract of PBS showed little affection on the proliferation of THP-1 cells after seventy-two hours＇ incubation and 50% concentration of inhibition was not visible.The expression of Bax /Bcl-2 changed significantly（P〈0.05）,however,the variations of Smac /IAP-1 were tiny（P〉0.05）.The results of chromosomal aberration tests were in normal range at the same time.Conclusions PBS do lightly affect on the biological characteristics of THP-1 cells and it can be a promising biodegradable polymer for sternal fixation because of excellent biocompatibility.

关 键 词：细胞增殖 细胞凋亡 聚丁二酸丁二醇酯 Bax Bcl-2 SMAC IAP-1 染色体畸变试验 

分 类 号：R318.08[医药卫生—生物医学工程]