右美托咪定对血管内皮细胞氧化应激损伤的影响  

Effects of dexmedetomidine on human vascular endothelial cells injury induced by oxidative stress

作　　者：苏玲[1] 屠伟峰[1] 陈茜 欧阳惠碧[1] 郄文斌[1] 

机构地区：[1]南方医科大学附属广州军区广州总医院麻醉科、全军临床麻醉中心,广州市510010 [2]广州市妇女儿童医疗中心麻醉科,510623

出　　处：《实用医学杂志》2013年第9期1397-1399,共3页The Journal of Practical Medicine

基　　金：广州市科技计划项目(编号:2008Z1-E421)

摘　　要：目的:探讨右美托咪定(dexmedetomidine)对过氧化氢(H2O2)诱导损伤的人脐静脉血管内皮细胞(HUVECS)的影响。方法:体外培养HUVECS,随机分为6组:对照组(NC组)、H组、D组、DH组、DHY组和HY组。200μmol/LH2O2建立血管内皮细胞氧化应激损伤模型,采用倒置显微镜观察各组细胞形态差别;MTT法测定各组细胞存活率;Hochest染色和流式细胞术检测细胞凋亡情况;测定各组细胞超氧化物歧化酶(SOD)和丙二醛(MDA)。结果:与NC组比较,H组细胞生存率降低(P<0.01),细胞凋亡率升高(P<0.05),SOD活力下降(P<0.05),MDA含量增加(P<0.05);而DH组相对于H组,细胞生存率升高,细胞凋亡率降低,SOD活力升高,MDA含量减少(均P<0.05)。结论:右美托咪定对H2O2诱导损伤的血管内皮细胞具有一定的保护作用。Objective To investigate the effects of dexmedetomidine（DEX）on human umbilical vein endothelial cells（HUVECS）injury induced by hydrogen peroxide（H2O2）.Methods HUVECS were cultured in vitro and randomly divided into 6 groups：NC group,H group,D group,DH group,DHY group and HY group.HUVECs oxidative stress injury models were constructed by 200 μmol /L of H2O2.The differences in cell morphology of HUVECS were observed under inverted microscope and the viablity of HUVECS was determined by the methyl thiazolyl tetrazolium（MTT）assay.Hochest staining and flow cytometry were used to detect HUVECS apoptosis.The activity of superoxide dismutase（SOD）and contents of malonadehyde（MDA）in the supernatant of HUVECS were determined by spectra photometric assay.Results As compared with those in NC group and DH group,the viablity of HUVECS and the activity of SOD were decreased（P〈0.01 and P〈0.05;both P〈0.05,respectively）,the apoptosis rate and the content of MDA were increased（both P〈0.05;both P〈0.05,respectively）.Conclusion Dexmedetomidine can inhibit the injury of HUVECS induced by H2O2 and has a protective effect.

关 键 词：右美托咪定 HUVECS H2O2 育亨宾 

分 类 号：R96[医药卫生—药理学]