1990～1997年云南IDUs HIV-1B亚型分离株膜蛋白V3环顶端四肽基因序列的变化趋势  

Variation trend of the tetrapeptide on the tip of V3 loop of 62 HIV-1 strains isolated in IDU of Yunnan from 1990 to 1997

作　　者：吴虹[1] 王斌[1] 方荣[1] 路永波 钱冬萌[1] 曾毅[2] 

机构地区：[1]青岛大学医学院分子病毒学实验室 [2]中国预防医学科学院病毒学研究所

出　　处：《中华微生物学和免疫学杂志》2000年第5期462-464,共3页Chinese Journal of Microbiology and Immunology

基　　金：卫生部科研基金!(96 135 3);山东省优秀中青年科学家奖励基金!(97314 4 32 )

摘　　要：目的　观察云南静脉药瘾 (IDUs)HIV 1分离株env基因V3环顶端四肽氨基酸和相应核苷酸序列随时间推移的变化。方法　根据 1990～ 1997年间 6 2株HIV 1分离株env基因C2 V3区DNA序列 ,对HIV 1膜蛋白V3环顶端四肽基因序列 (基序 )及其编码核苷酸进行分析 ,并探讨其随时间推移的变化趋势。结果　 1990～ 1997年间 6 2株HIV 1毒株膜蛋白V3环顶端四肽基序有程度不同的氨基酸变异 ,主要表现在第四位氨基酸的改变 ,变异呈现一定的趋向性。 6 2株云南标本中有 46例(74 2 % )为GPGR ,10例 (16 1% )为GPGQ ,4例 (6 4% )为GPGK ,另有 2例 (3 3% )为GPER且四肽基序编码核苷酸的变异主要表现为A→G的改变。结论　 1990～ 1997年这一时期内 ,云南IDUs特定感染者范围内HIV 1分离株膜蛋白V3环顶端四肽序列已表现出较大的趋向性 ,符合HIVObjective To investigate the variation trend of the amino acid and the corresponding nucleotide sequences of the tetrapeptide on the tip of the V3 loop of HIV 1 strains from intravenous drug users (IDUs) in Yunnan as time goes by. Methods According to the nucleotide sequences of the C2 V3 region of 62 HIV 1 strains isolated from intravenous drug users (IDUs) of Yunnan from 1990 to 1997, the nucleotide sequences of the tetrapeptide on the tip of the V3 loop of HIV 1 envelope protein were analysed. The variation trends with time were discussed. Results The tetrapeptides on the tip of the V3 loop of 62 Yunnan HIV 1 strains showed different amino acid variation with the major one at the fourth amino acid. Moreover, the variation tend to be in a similar pattern. Among the 62 isolated strains, 46 (74.2%) showed the GPGR tetrapeptide motif, 10 (16.1 %) GPER motif, 4 (6.4%) GPGK motif and 2 (3.3%) GPER motif respectively. The variation at corresponding nucleotide sequence of the tetrapeptide motif was A→G. Conclusion In the period of 1990 1997,the tetrapeptides on the tip of the V3 loop of the HIV 1 strains from infected IDUs people in Yunnan are similar, and the results conform to the GPGR motif pattern of the international B subtype HIV 1SF 2 strain.

关 键 词：V3环顶端四肽基因序列 静脉药瘾 艾滋病毒 

分 类 号：R392[医药卫生—免疫学]