酶联免疫反应加速仪在巨细胞病毒IgG抗体检测中的应用  

The value of enzyme-linked immune response accelerometer in detecting cytomegalovirus immunoglobulin G

作　　者：耿珍[1] 刘铭[1] 

机构地区：[1]郑州大学公共卫生学院,郑州450002

出　　处：《中国计划生育学杂志》2013年第5期340-342,共3页Chinese Journal of Family Planning

摘　　要：目的:探讨酶联免疫反应加速仪在巨细胞病毒(CMV)IgG检测中的临床应用价值。方法:取标准品、阴性和阳性对照血清、已知结果的临床血清标本,使用酶联免疫收附试验(传统法)和酶联免疫反应加速仪法(加速法)进行巨细胞IgG检测,比较观察加速法检测CMVIgG的重复性、灵敏度和特异度是否符合试剂盒标准。结果:线性相关性分析加速法和传统法定量结果呈正相关关系。加速法的变异系数为10%～2.1%,灵敏度为98.2%,特异度为96.3%,均在规定范围内。结论:加速仪用于CMVIgG检测,各项指标均符合试剂盒要求,可在临床检测中使用。Objective： To explore the value of the enzyme - linked immune response accelerometer in detecting cytomegalovir-us immunoglobulin G （ CMV IgG）. Methods ： The CMV IgG measurement was conducted with enzyme - linked immunosorbent assay （the traditional method） and enzyme-linked immune response accelerometer testing （the accelerating method） respec-tively using standard products, negative and positive control serum and clinical serum samples with known results. The repeat-ability, sensitivity and specificity of the two methods for detection of CMV IgG were compared. Results ： The quantitative result of linear correlation analysis showed the accelerating method and the traditional method were positively correlated. For the ac-celerating method, the variation coefficient, sensitivity and specificity were 10%-2.1%, 98.2% and 96.3%, respectively, which were all in the prescribed scope. Conclusion： Enzyme - linked immune response accelerometer can be used for the de-tection of CMV IgG in the clinical practice.

关 键 词：巨细胞病毒IgG 酶联免疫吸附试验 酶联免疫反应加速仪 

分 类 号：R446.6[医药卫生—诊断学]