鞘内注射曲马多对福尔马林炎性疼痛大鼠脊髓c-fosmRNA及蛋白质表达的影响  

Effect of Intrathecal Injection of Tramadol for Formalin Inflammatory Pain in Rat Spinal Cord on C-fos mRNA and Protein Expression

作　　者：吴仪[1] 李建明[1] 刘博[1] 

机构地区：[1]长沙医学院外科教研室,湖南长沙410219

出　　处：《医学临床研究》2013年第4期635-637,共3页Journal of Clinical Research

基　　金：湖南省教育厅科研课题一项（项目编号-09C156）

摘　　要：【目的】探讨预先鞘内注射曲马多对福尔马林炎性疼痛大鼠脊髓c—fosiTIRNA及蛋白质表达的影响。【方法】选取鞘内置管成功的32只雄性大鼠随机分成四组，每组8只，分别注射生理盐水（NS组），12．5ug／h曲马多（T1组），25ug／h曲马多（T2组），50ug／h曲马多（T3组）。制备福尔马林炎性疼痛大鼠模型，取模型大鼠脊髓L叫～I，6阶段脊髓做c—losmRNA的半定量RT—PCR和Western-blotting检测，观察大鼠脊髓c—los的mRNA及蛋白质表达量的变化。【结果】Ns组中的c—fosmRNA表达水平1.34±0．40明显高于T2组0．69±0．40（P〈0．01），高于T1组1．12±0．50，但比较差异无统计意义（P〉0．05）；T3组0．41±0．13显著低于T2组，T2组显著低于T1组，其差异均有统计学意义（P〈0．01）。NS组中的c—los蛋白表达水平1．20±0．50明显高于T2组0．64±0．40（P〈0．01），高于T1组0．98±0．38，但比较差异无统计意义（P〉0．05）；T3组0．27±0．09显著低于T2组，T2组显著低于T1组，其差异均有统计学意义（P〈O．01）。【结论】鞘内注射曲马多均能抑制大鼠脊髓背角c—fos的1TIRNA和蛋白质的表达量；不同剂量的曲马多对c—los表达量的影响不同，但剂量低时对c—fos表达无影响。[Objective]To explore the effect of intrathecal pre-injection of tramadol for formalin inflammatory pain in rat spinal cord on c-fos mRNA and protein expression. [Methods] A total of 32 male rats with successful intrathecal injection were randomly divided into four groups with 8 in each. All rats were injected with normal sa- line（Group NS）, tramadol 12. 5t2g/h（Group T1）, tramadol 25/~g/h（Group T2） and tramadol 50/2g/h （Group T3）, respectively. Rat model of formalin inflammatory pain was established. Spinal cord L4-L6 was taken to de- tect the expression of c-los mRNA by using semi-quantitative RT-PCR and western-blotting. The changes of e-los mRNA and protein expression in rat spinal cord were observed. [Results]The expression level of c-los mRNA in Group NS was significantly higher than that in Group T2（1.34 ! 0.40 vs. 0.69 i 0.40, P 〈0.01） and Group TI（1.34 ~ 0.40 vs. 1.12~0.50, P ~0.05） without statistical significance. The expression of c-los mRNA in Group T3 was significantly lower than that in Group T2（0.41 i 0.13 vs. 0.69 ~ 0.40）, and that in Group T2 was significantly lower than that in Group TI（0.69 ± 0.40 vs. 1.12 ± 0.50）, and there were significant differ- ences（ P 〈0.01）. The expression of C-los protein in Group NS was significantly higher than that in Group T2（1. 20 ± 0.50 vs. 0.64 ± 0.40, P〈0.01） and Group T1（1.20± 0.50 vs. 0.98 ：k 0.38, P）0.05） without statis- tical significance. The expression level of c-los mRNA in Group T3 was significantly lower than that in Group T2 （0.27 --/- 0.09 vs. 0.64 --4- 0.40）, and that in Group T2 was significantly lower than that in Group TI（0.64 ~ 0. 40 vs. O. 98 ~ O. 38）, and there was significant difference（ P〈0.01）. [Conclusion] Intrathecal injection of tram- adol can inhibit c-los mRNA and protein expression of rat spinal cord dorsal horn. Different dosage of tramadol has different effect on c-los expression, but low dose has no effect.

关 键 词：曲马朵 投药和剂量 疼痛测定 疾病模型 动物 基因 los RNA 信使 蛋白质类 大鼠 

分 类 号：R916.61[医药卫生—药学]