桃叶绿体全序列微卫星分布规律研究  

Distribution Analysis of Chloroplast Microsatellite in Chloroplast DNA in Peach(Prunus persica L.)

作　　者：魏潇[1] 章秋平[1] 刘威生[1] 杨巍[1] 

机构地区：[1]辽宁省果树科学研究所,辽宁熊岳115009

出　　处：《辽宁农业科学》2013年第2期5-8,共4页Liaoning Agricultural Sciences

基　　金：国家自然科学基金项目(31071767);公益性行业(农业)科研专项(201003058)

摘　　要：对桃叶绿体基因组全序列中微卫星的分布规律进行了全面分析。结果发现,cpSSR主要分布在大单拷贝区和小单拷贝区,以完全重复为主。单碱基重复最多,占总数的75.68%,二碱基重复占21.62%,单碱基重复主要以(A)n和(T)n为主,二者共占总数的67.57%,二碱基重复主要是(AT)n或(TA)n,占总数的20.47%。单碱基重复(A)n和(T)n的重复次数分别以11次和10次最多,而二碱基重复(AT)n、(TA)n和(TC)n的重复次数均为5～6次。研究结果为开发桃李属植物叶绿体通用引物提供了理论依据。The distribution of chloroplast microsatellite （cpSSR） in the chloroplast complete sequence of peach （Prunus persica L. ） was analysed. The results showed that the chloroplast SSR of peach mainly distributed in large single-copy region and small single-copy region and most of cpSSR were completely repeat. Mononucleotide repeat motif and dinucleotide repeat motif accounted for 75.68% and 21.62% of the total, respectivly. Repeat A and T were the main base in mononucleotide motif SSRs, accounting for 67.57%. Dinucleotide repeat motif mainly were （ AT）, or （TA） n, accounting for 20.47%. The mostly repeat number of （A） n and （T）, were 11 and 10, repeat number of （AT）., （ TA）, and （ TC）, were 5 or 6. These results were available for develop general primers from chloroplast DNA in Prunus.

关 键 词：桃 叶绿体DNA 微卫星 

分 类 号：Q754[生物学—分子生物学]