5-氨基酮戊酸光动力疗法对白念珠菌抑制效应的实验研究  

Inhibitory effect of 5-aminolevulinic acid photodynamic therapy on Candida albicans

作　　者：梁义[1] 卢丽明[1] 陈勇[1] 尹琦[1] 

机构地区：[1]柳州市柳铁中心医院皮肤科,广西柳州545007

出　　处：《海南医学》2013年第10期1405-1407,共3页Hainan Medical Journal

基　　金：广西区卫生厅课题(编号:Z2012552)

摘　　要：目的评估5-氨基酮戊酸光动力疗法(5-ALA-PDT)在体外对白念珠菌的抑制效应。方法以96孔细胞培养板为实验载体,加入白念珠菌悬液,分为治疗组和4个对照组,以氦氖激光为激发光源,ALA为光敏剂,使用DMSO作为ALA的溶剂兼穿透增强剂,进行ALA-PDT。结果治疗组白念珠菌的生长受到抑制,其MIC值为500mg/ml(50%),ALA浓度在200mg/ml(20%)时,开始使治疗组白念珠菌的生长受到抑制,且抑制作用对ALA的浓度呈剂量依赖关系。而对照组中白念珠菌的生长未受到抑制,四组的白念珠菌的CFU数量变化无明显差异。结论按本实验所使用的方法进行的ALA-PDT能有效地抑制白念珠菌。Objective To evaluate the inhibitory effect of 5-aminolevulinic acid Photodynamic therapy on Candida albicans in vitro. Methods 96-well cell culture plates were used for experimental carriers, and Candida al- bicans suspension was added to these wells, which were divided into experimental group and four control groups. With He-Ne laser as the excitation source, ALA as photosensitizer, DMSO as penetration enhancer and a solvent, ALA-PDT was performed. Results The growth of Candida albicana was inhibited in experimental group, with MIC of 500 mg/ml （50%）. At concentration of 200 mg/ml （20%）, ALA began to inhibit the growth of Candida albicans in experimental group, and ALA inhibited the growth of Candida albicans in a concentration-dependent way. The growth of Candida albicans was not inhibited in four control groups, and the CFU of Candida a Ibicans showed no statistically significant difference between the groups. Conclusion ALA-PDT can effectively inhibit the growth of Candida aIbicans.

关 键 词：5-氨基酮戊酸 光动力疗法 白念珠菌 抑制 

分 类 号：R379.4[医药卫生—病原生物学]