T细胞亚群偏移与药物流产小鼠子宫出血量相关性研究  

The research of correlation between T-cell subsets and uterine bleeding volume of medical abortion mice

作　　者：刘海静[1,2] 鞠红梅[3] 刘淑娟[3] 王丽[1] 赵向忠[1] 李霞[1] 

机构地区：[1]济南大学山东省医学科学院医学与生命科学学院,山东济南250062 [2]济宁医学院基础学院,山东济宁272067 [3]山东中医药大学附属医院,山东济南250011

出　　处：《济宁医学院学报》2013年第2期89-93,共5页Journal of Jining Medical University

基　　金：国家自然科学基金项目(No.30901918);山东省科技发展计划(2012GSF11840);山东省博士基金项目(No.BS2009YY041);山东省自然科学基金项目(No.ZR2010HQ066);山东省计生委项目(No.[2009]8);山东省中管局项目(No.2009-173);济南市青年科技明星计划项目(济科计[2012]16号;20120135)

摘　　要：目的探讨药物流产(简称药流)小鼠局部母胎界面T淋巴细胞亚群(Th1/Th2/Th17/Treg)偏移状态与子宫出血量相关性,为减轻药流后子宫出血寻找有效途径。方法孕鼠30只,随机分为正常妊娠组和米非司酮(RU486)大(1.5mg/kg/d)、小剂量组(0.88mg/kg/d),每组10只。改良碱性正铁血红素法检测子宫出血量,流式细胞术检测子宫组织Th1(CD3+CD4+IFNγ+)、Th2(CD3+CD4+IL-4+)、Th17(CD3+CD4+IL-17A+)、Treg(CD4+CD25+Foxp3+)亚群比例。结果 RU486小剂量组小鼠子宫出血量明显大于大剂量组(P<0.05);与正常妊娠组比较,RU486药流小鼠母胎界面Th1、Th17亚群比例升高(P<0.05),Th2、Treg亚群比例降低(P<0.05),Th1/Th2、Th17/Treg比值显著升高(P<0.05);药流小鼠子宫出血量与Th1、Th17亚群比例及Th1/Th2、Th17/Treg比值呈显著负相关(P<0.05),与Th2、Treg亚群比例呈显著正相关(P<0.05)。结论诱导Th1/Th2/Th17/Treg亚群偏移是RU486药流机制之一,RU486诱导的Th1、Th17偏移状态与子宫出血量间存在密切相关性,靶向诱导Th1、Th17亚群偏移的方法可能成为减轻药流子宫出血的有效途径。Objective To investigate the correlation between uterine bleeding volume and shift state of T lym- phocyte subsets（Thl/Th2/ThlT/Treg）at the maternal-fatal interface of medical abortion mice and to find an effec- tive way to reduce the uterine bleeding during medical abortion. Methods 30 pregnant mice were divided random- ly into normal pregnancy group,mifepristone （RU486） high-dose group （1.5 mg/kg/d） and low-dose group （0.88 mg/kg/d） ,and 10 mice in each group. The uterine bleeding volume of model mice was detected using alkaline hem- atin photometric method,and the proportion of Thl （CD3＋ CD4＋ IFN7＋ ）, Th2 （CD3＋ CD4＋ IL-4＋ ）, Thl7 （CD3＋ CD4＋ IL-17A＋ ） and Treg（CD4＋ CD25＋ Foxp3＋ ） of uterine tissue were measured by flow cytometry.Results The uterine bleeding volume of medical abortion mice in RU486-1ow-dose group was more grater than that of high-dose group （P-~0.05）. Compared to normal pregnant-mice, the percentage of Thl and Thl 7 subsets at the maternal-fe- tal interface in medical abortion mice increased; the percentage of Th2 and Treg subsets decreased; the ratios of Thl/Th2 and ThlT/Treg increased remarkably （P~ 0.05）. The volume of uterine bleeding of medical abortion mice was negatively related to the proportions of Thl and Th17 cells as well as the ratios of Thl/Th2 cells and Thl 7/Treg cells （P〈~0.05） ,and positively related to the proportions of Th2 and Treg cells （P^0.05）. Conclu- sion Induction of Thl/Th2/Thl7/Treg subsets skew is one of the mechanisms during RU486-induced abortion. The state of T cell subsets skew in RU486 medical abortion was closely related to the uterine bleeding volume. Tar-geting to induce T cell subsets skew may become an effective way to reduce the uterine bleeding during medical a- bortion.

关 键 词：药物流产 子宫出血 T淋巴细胞 TH1 TH2 Th17 TREG 

分 类 号：R392.12[医药卫生—免疫学]