载体中tet^R和amp^R抗性检验基因及蓝白筛选原理  

tet^R and amp^R Resistance-detecting Genes in Vector and Blue-white Filtering Principle

作　　者：孙伟男[1] 张玉[1] 

机构地区：[1]内蒙古农业大学动物科学学院,内蒙古呼和浩特010018

出　　处：《畜牧与饲料科学》2013年第4期17-18,共2页Animal Husbandry and Feed Science

摘　　要：转基因过程中可能会带有一些不期望的分子:未正确连接的载体、PCR过程中的DNA碎片、没有插入DNA片段的载体、其他重组DNA分子、插入不正确的片段等,这些都会给转基因克隆带来一定的麻烦。经过研究发现,一些载体可以携带不同的抗性检测基因,通过合适的筛选步骤可以把所需要的克隆与不需要的非目的克隆区分开,达到纯化DNA的目的。介绍了几种载体中的检验基因及其原理。Transgenic process may be with some undesirable molecules, such as error-connected carrier, DNA fragments in the PCR process, vector without inserted DNA fragment, other recombinant DNA molecule and insert incorrect fragment. These will bring some troubles to transgenic cloned. Studies showed that some carrier may carry different resistance-detecting genes, and the require cloning and unwanted non-purpose cloning region could be separated through a suitable screening step to achieve the object of purified DNA. The test gene and its principle of three carriers were described.

关 键 词：转基因 转基因载体 抗性检测基因 分离纯化DNA 

分 类 号：Q78[生物学—分子生物学]