兔骨髓来源的内皮祖细胞体外培养及鉴定  

Culture and Identification of Endothelial Progenitor Cells from Rabbit Bone Marrow in Vitro

作　　者：刘丹平[1] 赵辉[2] 齐鑫[2] 

机构地区：[1]辽宁医学院附属第一医院 [2]辽宁医学院,辽宁锦州121001

出　　处：《辽宁医学院学报》2013年第2期6-8,I0001,共4页Journal of Liaoning Medical University (LNMU) Bimonthly

基　　金：辽宁省卫生厅"百千万人才工程"资助项目;项目编号:2010921045

摘　　要：目的在体外获取兔骨髓来源的内皮祖细胞。方法 取1 w龄兔的长骨骨髓,通过密度梯度离心结合差速贴壁法分离培养内皮祖细胞。结果 获取较多目的细胞,经鉴定符合内皮祖细胞的形态特征,能够摄取并结合DIL-ac-LDL及FITC-UEA-1。结论 密度梯度离心结合差速贴壁法可以得到较多骨髓来源的内皮祖细胞。Objective To get the endothelial progenitor cells from Rabbit Bone Marrow in Vitro. Methods Long bone marrow of lw rabbit was taken, whose endothelial progenitor cells were separated and cultured by the method of densi- ty gradient centrifugation combined with differential adhesion. Results More objective cells can be obtained, which proved to be in accordance with the morphological characteristics of endothelial progenitor cells, and can absorb and com- bine with DIL - ac - LDL and FITC - UEA - 1. Conclusion More endothelial progenitor cells from bone marrow can be obtained by the method of density gradient centrifugation combined with differential adhesion.

关 键 词：内皮祖细胞 密度梯度离心 兔 骨髓 

分 类 号：Q813.1[生物学—生物工程]