阿魏酸钠抑制脂多糖引起的星形胶质细胞IL-1β的表达  

Effect of Sodium Ferulate on the Expression of Interleukin-1β Induced by Lipopolysaccharide in Cultured Astrocytes

作　　者：黄军[1] 张有志[1] 闫恩志[2] 

机构地区：[1]辽宁医学院 [2]辽宁医学院药理学教研室,辽宁锦州121001

出　　处：《辽宁医学院学报》2013年第2期14-16,共3页Journal of Liaoning Medical University (LNMU) Bimonthly

基　　金：辽宁省教育厅科学研究一般项目;项目编号:L2012310;辽宁医学院"奥鸿大学生创新课题基金"项目

摘　　要：目的研究阿魏酸钠(SF)对脂多糖(LPS)引起的培养星形胶质细胞(AC)白细胞介素-1β(IL-1β)表达的抑制作用及机制。方法 将培养星形胶质细胞分为对照组、LPS组、SF(50,100,200μmol·L-1)组。细胞成熟后分组给药处理,ELISA法测定培养液中IL-1β表达,Western蛋白印迹法检测细胞IL-1β、磷酸化JNK1和磷酸化C-Jun蛋白表达。结果 星形胶质细胞在LPS(10 mg·L-1)刺激下IL-1β的释放量、IL-1β蛋白以及磷酸化JNK1、磷酸化C-Jun蛋白表达水平较对照组明显增高(P<0.01)。应用SF(50,100,200μmol·L-1)预处理6 h明显降低培养液中IL-1β含量,抑制星形胶质细胞IL-1β、磷酸化JNK1和磷酸化C-Jun蛋白表达水平。结论 SF可能通过下调JNK信号通路抑制星形胶质细胞IL-1β表达。Objective To investigate whether Sodium Ferulate （SF） could protect astrocytes from LPS -induced ex- pressions of IL - 1βand the mechanisms responsible for this protective effect. Methods The primary cultured astrocytes were exposed to LPS 10 mg. L^-1 for 24 h after with and without pretreatment with various concentrations of SF （50, 100, 200 μmol . L^- 1 ） for 6 h. The supernatants of astrocytes were collected and proinflammatory cytokines IL - 1β was deter- mined by ELISA technology, and western blot was performed to observe the level of IL - 1β, p - JNK1 and p - C - Jun in cultured astrocytes. Results Cultured astrocytes treated with LPS significant increased the level of IL - 1β p - JNKI and p - C - Jun, and the release of IL - 1. SF （50, 100, 200 μmol. L……-1 ） inhibited LPS - induced increase in expression of IL-1β, and p - JNK1 and p - C - 3un in cultured astrocytes. Conclusion SF can inhibit the expression of IL - 1βinduced by LPS in cultured astrocytes through the suppression of JNK signal transduction pathway activity.

关 键 词：阿魏酸钠 脂多糖 星形胶质细胞 炎症反应 IL-1Β 

分 类 号：R971[医药卫生—药品]