HBV相关性肝病患者肝组织中HBxAg、Bax及Bcl-2的对比研究  

Comparative study of HBxAg and Bax and Bcl-2 in liver tissue from patients with HBV-related liver disease

作　　者：李程[1] 王永康 杜磊[1] 刘伟[1] 俎燕会[1] 王昌源[1] 

机构地区：[1]山东大学医学院,山东大学附属传染病医院,山东济南250021 [2]山东省省立医院病理科,山东济南250021

出　　处：《中国病原生物学杂志》2013年第4期293-297,共5页Journal of Pathogen Biology

基　　金：济南市科学技术局资助项目(No.200807033-1)

摘　　要：目的对比观察HBxAg、凋亡相关蛋白Bax和Bcl-2在乙肝相关性肝病患者肝组织中的表达,探讨HBxAg在相关肝病进展中的作用。方法收集38例慢性HBV感染(CHB)、20例乙肝肝硬化(LC)和20例HBV相关性肝细胞癌(HCC)患者的肝组织标本,采用免疫组织化学染色法检测HBxAg、Bax和Bcl-2的表达。结果 3组患者肝组织中HBxAg阳性率分别为71.1%、60.0%、65.0%,差异无统计学意义(χ2=0.754,P>0.05)。3组患者Bax蛋白阳性率差异有统计学意义(χ2=14.190,P<0.01),其中HCC、CHB组Bax阳性率均高于LC组(χ2=10.417,12.214,P均<0.01)。LC组患者肝细胞Bcl-2阳性率高于CHB组(χ2=5.968,P<0.05),HCC组患者肝组织单核淋巴细胞Bcl-2阳性率高于CHB和LC组(χ2=14.872,10.000,P均<0.01)。HBxAg表达强度与Bax及Bcl-2均无相关性(r分别为0.100和0.197,P均>0.05);高、中病毒载量组HBxAg阳性率均高于低病毒载量组(χ2分别为16.800和6.930,P均<0.05)。结论 HBxAg不直接调节Bax及Bcl-2的表达,可能通过影响其作用途径来调节细胞凋亡。CHB患者肝细胞Bax表达占优势,以凋亡为主;LC患者Bcl-2表达占优势,以抑制凋亡为主。肝组织单核淋巴细胞中Bcl-2高表达可能与疾病进展有关。高病毒载量通过HBxAg的大量表达促进肝癌的发生。Objectives To compare the expression of HBxAg and the apoptosis related proteins Bax and Bcl 2 in liver tissue from patients with hepatitis B virus （HBV）-related liver disease and to explore the function of HBxAg in the pro- gression of disease. Methods Liver tissue samples were collected from 38 patients with chronic HBV infection （CHB）, 20 with HBV-related liver cirrhosis （LC） and 20 with hepatocellular carcinoma （HCC）. Immunohistochemical staining was used to detect the expression of HBxAg, Bax and Bcl-2 in liver tissue from the three groups. The chi-square test was used for group comparison and Spearman rank correlation analysis was used for correlation analysis. Results Liver tis- sue of the three groups was positive for HBxAg at a rate of 71. 1%, 60.0%, and 65.0%, respectively, and the differ- ences in positivity were not statistically significant （ x^2= 0. 754, P〉 0.05）. The 3 groups had statistically significant differences （ x^2=14. 190, P〈0.01） in positivity for Bax. Among them, patients with HCC and patients with CHB had greater positivity for Bax than did patients with LC （ x^2 =10. 417, 12. 214, P〈0.01）. Patients with LC tested positive for Bcl-2 at a higher rate than CHB patients （ x^2 =5. 968, P〈0.05）. Compared to patients with CHB and LC, patients with HCC tested positive for Bcl-2 in mononuclear lymphocytes at a higher rate （x^2 of 14. 872 and 10. 000, respectively, P〈0.01）. The intensity of HBxAg expression had no correlation with Bax and Bcl-2 （r=0. 100, 0. 197, P〈0.05）. Pa tients with high or medium viral loads tested positive for HBxAg at a higher rate than patients with a low viral load （ x^2 =16. 800, 6. 930, P〈0.05）. Conclusion HBxAg does not directly modulate Bax and Bcl-2 expression and may regu- late cell apoptosis by affecting their routes of action. Bax predominated in liver cells from patients with CHB and thus pri- marily led to apoptosis while Bcl-2 predominated in patients with LC and inhibited apoptosis. A high level of Bc

关 键 词：HBXAG 凋亡 BAX BCL-2 免疫组织化学染色 

分 类 号：R373.21[医药卫生—病原生物学]