旋毛虫肌幼虫囊包单染与复染方法比较研究  

A comparative study of single staining and duplicate staining of specimens of encapsulated Trichinella spiralis larvae

作　　者：李丹[1] 牛利娜[1] 皮本伟[1] 杨丁[1] 张运生[2] 

机构地区：[1]河南大学医学院临床学院,河南开封475000 [2]河南大学公共卫生研究所,河南开封475004

出　　处：《中国病原生物学杂志》2013年第4期350-351,354,F0003,共4页Journal of Pathogen Biology

基　　金：河南大学大学生创新性实验资助课题(No.2010-10NB032)

摘　　要：目的比较单染和复染染色方法制作旋毛虫肌幼虫囊包标本的效果。方法单染,醋酸明矾卡红染色液染色:①染色24h后,分色;②染色24h,不分色。复染,醋酸明矾卡红染色液与固绿染色液染色:③醋酸明矾卡红染色24h后,分色;固绿染色20s;④醋酸明矾卡红染色24h,不分色;固绿染色20s。结果方法①制作的标本囊包轮廓清晰,囊内幼虫体态明显;方法③制作标本,囊包与周围肌细胞分界明显,立体感强;囊内幼虫、囊壁内层、囊壁外层相互之间层次分明,特征典型。结论方法①和③制作的标本,染色效果好,易于观察。Objective To compare the effectiveness of single staining and duplicate staining of specimens of Trichinella spiralis encapsulated larvae. Methods With single staining, the specimens were stained with acetate alum carmine stai- ning solution： 1） specimens were stained for 24 h and then color was fixed or 2） specimens were stained for 24 h without color fixation. With duplicate staining, specimens were double-stained with acetate alum carmine staining solution and fast green staining solution：3） specimens were stained for 24 h with acetate alum carmine staining solution, color was fixed, and then specimens were stained for 20 s with fast green staining solution or 4） specimens were stained for 24 h with ace- tate alum carmine staining solution without color fixation and then stained for 20 s with fast green staining solution. Re- sults Specimens stained using 1） had clearly defined capsule edges and encapsulated larvae were readily visible. The re- sults also showed that specimens stained using 3） had clearly distinguishable larvae, an internal cyst layer, and an exter- nal cyst layer and characteristic appearance. Conclusion Specimens were effectively stained and visualized using 1） and 3）.

关 键 词：旋毛虫 肌幼虫囊包 染色 

分 类 号：R383.15[医药卫生—医学寄生虫学]