大孔吸附树脂分离纯化莱菔子水溶性生物碱的工艺研究  

Purification of Alkaloids from Semen Raphani by Macroporous Adsorption Resin

作　　者：李民[1] 周洪雷[1] 丁臻[1] 李运伦[2] 史玉霞[1] 郑德[1] 黄真[1] 

机构地区：[1]山东中医药大学,山东济南250355 [2]山东中医药大学附属医院,山东济南250011

出　　处：《辽宁中医杂志》2013年第5期974-976,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：山东省自然科学基金项目(ZR2010HM017);山东省教育厅科技计划项目(J105LL05);山东省科技发展计划项目(2008GG2NS02011);国家新药研发大平台(山东)(2009*ZX09301-013)

摘　　要：目的:研究大孔吸附树脂纯化莱菔子水溶性生物碱的工艺。方法:采用D101、HPD-600、AB-8大孔吸附树脂对莱菔子水溶性生物碱进行分离纯化,以莱菔子水溶性生物碱含量为考察指标。结果:AB-8大孔吸附树脂对莱菔子水溶性生物碱具有较大吸附量,洗脱方法:以6 BV蒸馏水去除杂质,以10 BV的30%乙醇洗脱,收集30%乙醇洗脱部分。结论:AB-8大孔吸附树脂综合性能较好,适合莱菔子水溶性生物碱的分离纯化。Objective：To study the technology for water-soluble Semen Raphani alkaloids by macroporous adsorption resin. Methods：DlO1, HPD -600 and AB -8 types of macroporous adsorption resins were used to separate and purify water-soluble al- kaloids. The yields and purities of the products were compared as indexes. Results ： AB - 8 type macroporous adsorption resin had optimal adsorption. The resin was washed with 6 BV of distilled water and alkaloids were desorbed by 10 BV of 30% ethanol. Con- clusion ：The AB -8 type macroporous adsorption resin shows a better comprehensive adsorption property and can be used to sepa- rate and purify water- soluble Semen Raphani alkaloids.

关 键 词：莱菔子 水溶性生物碱 树脂 分离纯化 

分 类 号：R284.2[医药卫生—中药学]