清肺除刺方免疫抗炎作用的实验研究及对血清sIL-2R的影响  

Immune anti-inflammatory effect of "Qingfei Chuci Decotion" and its effect on sIL-2R

作　　者：李燕娜[1] 李咏梅[1] 郭潋[1] 李晓睿[1] 

机构地区：[1]上海中医药大学附属龙华医院,上海200032

出　　处：《上海中医药杂志》2013年第5期85-88,共4页Shanghai Journal of Traditional Chinese Medicine

基　　金：上海中医药大学附属龙华医院科研基金资助项目(2010YM18);海派中医顾氏外科流派传承研究资助项目(ZYSNXD-CC-APGC-JD002)

摘　　要：目的观察清肺除刺方的免疫调节及抗炎作用。方法将70只BALB/C小鼠随机分为2,4-二硝基氟苯(DNFB)造模组32只,二甲苯造模组32只,正常对照组6只。DNFB造模组及二甲苯造模组的小鼠再分别随机分为清肺除刺方组、强的松组、丹参酮胶囊组、模型组,每组8只,分别予以相应的干预措施。观察各组小鼠的耳肿胀及病理状况,并检测小鼠血清可溶性白介素-2受体(sIL-2R)水平。结果①DNFB造模:与正常对照组比较,模型组小鼠左右耳片重量差差异有统计学意义(P<0.01);与模型组比较,各药物组小鼠左右耳片重量差差异有统计学意义(P<0.01);与强的松组比较,清肺除刺方组左右耳片重量差差异无统计学意义(P>0.05)。②二甲苯造模:与正常对照组比较,模型组小鼠左右耳片重量差差异有统计学意义(P<0.01);与模型组比较,各药物组小鼠左右耳片重量差差异有统计学意义(P<0.01);与强的松组比较,清肺除刺方组左右耳片重量差差异无统计学意义(P>0.05)。③与正常对照组比较,DNFB模型组小鼠血清sIL-2R水平明显升高,差异有统计学意义(P<0.01);与模型组比较,清肺除刺方组、强的松组、丹参酮胶囊组血清sIL-2R水平差异有统计学意义(P<0.01)。结论清肺除刺方具有确切的抗炎作用,并能在一定程度上抑制细胞免疫。Objective To observe the immunoregulatory and anti-inflammatory effects of ＂Qingfei Chuci Decoction＂. Methods Seventy BALB/C mice were randomized into two groups： 32 cases were modeled by 2, 4 December nitro fluorine benzene （DNFB） , and 32 cases were modeled by dimethylbenzene, 6 cases were in normal control group. The 64 models were randomized into ＂Qingfei Chuci Decoction＂ group, Prednisone group, Tanshinone Capsule group, model group, 8 mice in each group were treated by corresponding medicine. The ear swelling and pathological condition were observed, and the slL-2R level was detected. Results ① DNFB model ： there were significant difference in right-left ear weight difference between model group and normal control group （ P 〈 0.01 ） ; thel~ were significant difference hetween model group and medicine groups （ P 〈 0.01 ） ; no significant difference was found between Prednisone group and ＂ Qingfei Chuci Decoction＂ group （P 〉 0.05）. ② Dimethylbenzene model： there were significant difference in right-left ear weight difference between model group and normal control group （ P 〈0.01 ） ; significant difference was found between model group and the other groups （ P 〈 0.01 ） ; no significant difference was found between Prednisoue group and ＂Qingfei Chuci Decoction＂ group （ P 〉 0.05 ）. ③ Compared with normal control group, the slL-2R level was increased significantly in DNFB model group （ P 〈 0.01 ） ; no significant difference was found in slL-2R level in ＂Qingfei Chuci Decoction＂ group, Prednisone group, Tanshinone Capsule group （P 〉 0.05 ）. There were significant differences in sIL-2R level mnong model group, ＂Qingfei Chuci Decoction＂ group, Prednisoue group and Taushinone Capsule group （P 〈0.01 ）. Conclusion ＂Qingfei Chuci Decoction＂ has certain anti-inflammatory effect and can restrain the cellular immunity to some extent.

关 键 词：清肺除刺方 免疫 抗炎 实验研究 SIL-2R 

分 类 号：R285.5[医药卫生—中药学]