白血病抑制因子mRNA在早孕期小鼠子宫内膜的表达规律和组织学定位  

Expression of Leukemia Inhibitory Factor mRNA and Its Localization in the Endometrium of Early Pregnant Mouse

作　　者：张炜[1] 周剑萍[1] 刘银坤[2] 吴欣[2] 

机构地区：[1]上海医科大学妇产科医院研究所,上海200011 [2]中山医院肝癌研究所,上海200032

出　　处：《上海医科大学学报》2000年第4期280-282,共3页Journal of Fudan University(Medical Science)

基　　金：上海市卫生局科技发展基金资助项目! ( 964 2 9)

摘　　要：目的　研究白血病抑制因子 (LIF)mRNA在早孕期小鼠子宫内膜的表达规律及LIF蛋白的组织定位。方法　用RT PCR半定量法测定LIFmRNA的表达 ;用免疫组化ABC法测定LIF蛋白的组织学定位。结果　LIFmRNA在早孕期小鼠子宫内膜呈时序性规律表达 ,孕早期升高 ,第 4天达到高峰 ,比未孕期明显增高 (P <0 .0 1 ) ,此后下降 ,到孕第 7天降到孕前水平 ;LIF蛋白主要定位在子宫内膜腺上皮和腔上皮 ,且第 4天染色最深 ,间质细胞无LIF表达。结论　LIF的表达高峰与小鼠孕卵着床时间吻合。因此 ,LIF可能参与了着床的启动 ,LIF参与着床可能是通过自分泌和旁分泌作用的结果。Purpose To investigate the expression of leukemia inhibitory factor(LIF)mRNA and the location of LIF protein in the endometrium of early pregnant mouse. Methods The LIFmRNA expression was measured by RT?PCR,and the location of LIF protein was determined by immunohistochemistry(ABC method). Results The mRNA expression of LIF was increased during the period of early pregnancy,and went up to the highest level on day 4, then down to pre pregnant level on day 7.LIF was mainly located in luminal and glandular epithelium of endometrium,and the maximal staining of LIF on day 4.No staining was observed in the stromal cells. Conclusions The highest level of LIF on day 4 was coincided with the time of mouse blastocyst implantation.The result documented that LIF might be involved in onset of implantation.LIF may be involveed in embryo implantation via its paracrine/autocrine.

关 键 词：白血病抑制因子 子宫内膜 孕卵着床 MRNA 

分 类 号：R321.3[医药卫生—人体解剖和组织胚胎学] R737.33[医药卫生—基础医学]