化学发光法评价精液中的白细胞对精子质量的影响＊  

Effects of leukocytes on semen quality by chemiluminescence＊

作　　者：李文军[1] 刘玉林[2] 江峰[2] 汤晓峰[2] 吴斌[3] 杨志行[4] 施惠娟[3] 陈国武[2] 

机构地区：[1]江苏省江阴市人民医院泌尿外科,江阴214400 [2]上海集爱遗传与不育诊疗中心男科 [3]上海市计划生育科学研究所国家人口计生委计划生育药具重点实验室 [4]中国科学院上海生命科学研究院湖州营养与健康产业创新中心

出　　处：《中国男科学杂志》2013年第3期23-26,共4页Chinese Journal of Andrology

基　　金：由上海市科委2012年度“创新行动计划”基础研究重点项目（12JC1409400）和国家人口计生委C1类项目（C1.94）资助

摘　　要：目的探索用化学发光方法评价精液中过量存在的白细胞对精液质量的影响.方法将男性不育患者分为精液参数正常组,弱精子症组和少弱精子症组,通过化学发光方法直接检测精浆活性氧（ROS）水平,以及检测白细胞特异探针fMLP激惹白细胞后精液白细胞的ROS水平.结果精浆ROS和白细胞所产生的ROS水平在各组之间无显著差别,但用白细胞ROS指数平衡了精浆ROS水平和精子密度的影响后,白细胞ROS指数在弱精子症组和少弱精子症组均显著高于精液参数正常组.结论精液中若存在相对于精子数量过量的白细胞,且白细胞经化合物（fMLP）刺激后产生ROS水平的能力过强,可能是导致精子活动力低下的重要原因.Objective To evaluate the effects of leukocytes on semen quality. Methods Based on the parameters of semen analysis, the infertile males were divided into three groups such as the normal group, the athenospermia group and the oligozo-athenospermia group. Seminal ROS was detected by using chemiluminescence ,meanwhile the ability of WBC producing ROS in semen was determined by fMLP stimulating test. Results Among the three groups, no differences were found in the levels of ROS either in semen or produced by WBC. When the influence of seminal ROS and sperm concentration was normalized, the ROS index of WBC in both athenospermia group and oligozo-athenospermia groups were significantly higher than that in the normal group. Conclusion Relative to sperm concentration, high amount of seminal WBC and ROS level produced by WBC after fMLP stimulation might be the critical factors for decrease in the motility of sperm.

关 键 词：化学发光法 白细胞 精子 

分 类 号：R446.144[医药卫生—诊断学]