胰腺移植急性排斥反应中的细胞凋亡  

Cell apoptosis in pancreatic allografts during acute rejection

作　　者：李占京[1] 韩本立[1] 吴军[2] 

机构地区：[1]第三军医大学附属西南医院肝胆外科中心,重庆400038 [2]第三军医大学附属西南医院烧伤研究所,重庆400038

出　　处：《中华实验外科杂志》2000年第4期335-336,共2页Chinese Journal of Experimental Surgery

摘　　要：目的　观察胰腺移植急性排斥反应中的细胞凋亡变化。方法　对封闭群大鼠作单纯胰腺移植 (A组 )和胰脾联合移植 (B组 ) ,手术后第 3、7、12、2 0天每组各处死 5只 ,切取移植胰 ,石蜡包埋切片 ,原位DNA缺口末端标记 (TUNEL)法测定胰腺组织细胞凋亡。结果　A组术后第 3、7、12、2 0天胰腺细胞凋亡数分别为 (15 2 .5 5± 2 7.2 4)、(5 0 8.19± 117.83)、(70 1.33± 130 .17)和 (10 10 .5 6±114.6 9)个 /高倍视野 ;B组分别为 (12 4.87± 19.11)、(198.6 8± 31.99)、(492 .2 5± 93.0 1)和(6 49.80± 140 .91)个 /高倍视野。 2组各时点均显著高于正常胰腺。第 7、12、2 0天A组也显著高于B组。结论　胰腺移植急性排斥时细胞凋亡活性明显增高。Objective\ To study the changes of cell apoptosis in pancreatic allografts during acute rejection. Methods\ Non-inbred Wistar rats were used as donors and recipients. Two groups were designed: group A-simple pancreas transplantation, group B-combined pancreas-spleen transplantation. At 3, 7, 12 and 20 days after transplantation, 5 animals were sacrificed at each time point in each group. The pancreatic grafts were removed for apoptosic analysis by using TUNEL assay. Results\ The number of apoptosic cells in pancreatic grafts was 152.55±27.24), (508.19±117.83), (701.33±130.17) and (1010.56±114.69)/400×field in group A, and (124.87±19.11), (198.68±31.99), (492.25±93.01) and (649.80±140.91)/400× field in group B respectively at 3, 7, 12, and 20 days after transplantation. The number of apoptosic grafts in the two groups were significantly increased as compared with control group at each time point. The number in group A at 7,12 and 20 days after transplantation was also significantly higher than in group B. Conclusion\ During acute rejection, the number of apoptosic cells in pancereatic grafts is significantly increased.\;

关 键 词：大鼠 胰腺移植 细胞凋亡 急性排斥反应 

分 类 号：R657.5[医药卫生—外科学]