Culture observation and molecular phylogenetic analysis on the blooming green alga Chaetomorpha valida (Cladophorales, Chlorophyta) from China  

作　　者：邓蕴彦 汤晓荣 詹子锋 滕林宏 丁兰平 黄冰心 

机构地区：[1]Institute of Oceanology, Chinese Academy of Sciences [2]Graduate University of Chinese Academy of Sciences [3]Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education [4]Shantou University

出　　处：《Chinese Journal of Oceanology and Limnology》2013年第3期552-559,共8页中国海洋湖沼学报（英文版）

基　　金：Supported by General and Major Projects of the National Natural Science Foundation of China(Nos.31070185,31093440);the Science and Technology Project of Guangdong Province(Nos.2011B031100010,2012A020200007);the Talent Introduction Projects of Guangdong Province Universities and Colleges;the Team Project of Natural Science Foundation of Guangdong Province(No.S2011030005257);the Grant of the Key Laboratory of Integrated Marine Monitoring and Applied Technologies for Harmful Algal Blooms,SOA(MATHAB)(No.MATHAB20100301);the Science and Technology Project of Shantou City,China(No.2011-162)

摘　　要：The marine green alga Chaetomorpha valida fouls aquaculture ponds along the coastal cities of Dalian and Rongcheng, China. Unialgal cultures were observed under a microscope to determine the developmental morphological characters of C. valida. Results reveal that gametophytic filaments often produce lateral branches under laboratory culture conditions, suggesting an atypical heteromorphic life cycle of C. valida between unbranched sporophytes and branched gametophytes, which differs from typical isomorphic alternation of Chaetomorpha species. The shape of the basal attachment cell, an important taxonomic character within the genus, was found variable depending on environmental conditions. The 18S rDNA and 28S rDNA regions were used to explore the phylogenetic affinity of the taxa. Inferred trees from 18S rDNA sequences revealed a close relationship between C. valida and Chaetomorpha moniligera. These results would enrich information in general biology and morphological plasticity of C. valida and provided a basis for future identification of green tide forming algae.The marine green alga Chaetomorpha valida fouls aquaculture ponds along the coastal cities of Dalian and Rongcheng, China. Unialgal cultures were observed under a microscope to determine the developmental morphological characters of C. valida. Results reveal that gametophytic filaments often produce lateral branches under laboratory culture conditions, suggesting an atypical heteromorphic life cycle of C. valida between unbranched sporophytes and branched gametophytes, which differs from typical isomorphic alternation of Chaetomorpha species. The shape of the basal attachment cell, an important taxonomic character within the genus, was found variable depending on environmental conditions. The 18S rDNA and 28S rDNA regions were used to explore the phylogenetic affinity of the taxa. Inferred trees from 18S rDNA sequences revealed a close relationship between C. valida and Chaetomorpha moniligera. These results would enrich information in general biology and morphological plasticity of C. valida and provided a basis for future identification of green tide forming algae.

关 键 词：blooming green algae Chaetomorpha valida branch culture observation heteromorphic lifecycle molecular phylogeny 

分 类 号：S917.3[农业科学—水产科学]