Population genetics of Sargassum horneri (Fucales, Phaeophyta) in China revealed by ISSR and SRAP markers  

作　　者：于深辉 崇卓 赵凤娟 姚建亭 段德麟 

机构地区：[1]Key Laboratory of Experimental Marine Biology,Institute of Oceanology,Chinese Academy of Sciences [2]Graduate University of Chinese Academy of Sciences [3]Shandong Key Laboratory of Eco-environmental Science for Yellow River Delta,Binzhou University

出　　处：《Chinese Journal of Oceanology and Limnology》2013年第3期609-616,共8页中国海洋湖沼学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China(Nos.40618001,40976085);the Chinese Academy of Sciences and Guangdong Provincial Joint Projects(No.2009B091300086);Public Science and Technology Research Funds Projects of Ocean

摘　　要：Sargassum horneri is a common brown macro-alga that is found in the inter-tidal ecosystems of China. To investigate the current status of seaweed resources and provide basic data for its sustainable development, ISSR (inter simple sequence repeat) and SRAP (sequence related amplified polymorphism) markers were used to analyze the population genetics among nine natural populations of S. horneri. The nine studied populations were distributed over 2 000 km from northeast to south China. The percentage of polymorphic loci P% (ISSR, 99.44%; SRAP, 100.00%), Nei's genetic diversity H (ISSR, 0.107-0.199; SRAP, 0.100-0.153), and Shannon's information index I (ISSR, 0.157-0.291; SRAP, 0.148-0.219) indicated a fair amount of genetic variability among the nine populations. Moreover, the high degree of gene differentiation G st (ISSR, 0.654; SRAP, 0.718) and low gene flow N m (ISSR, 0.265; SRAP, 0.196) implied that there was significant among-population differentiation, possibly as a result of habitat fragmentation. The matrices of genetic distances and fixation indices (F st ) among the populations correlated well with their geographical distribution (Mantel test R=0.541 5, 0.541 8; P=0.005 0, 0.002 0 and R=0.728 6, 0.641 2; P=0.001 0, 0.001 0, respectively); the Rongcheng population in the Shandong peninsula was the only exception. Overall, the genetic differentiation agreed with the geographic isolation. The fair amount of genetic diversity that was revealed in the S. horneri populations in China indicated that the seaweed resources had not been seriously affected by external factors.Sargassum horneri is a common brown macro-alga that is found in the inter-tidal ecosystems of China. To investigate the current status of seaweed resources and provide basic data for its sustainable development, ISSR （inter simple sequence repeat） and SRAP （sequence related amplified polymorphism） markers were used to analyze the population genetics among nine natural populations of S. horneri. The nine studied populations were distributed over 2 000 km from northeast to south China. The percentage of polymorphic loci P% （ISSR, 99.44%; SRAP, 100.00%）, Nei＇s genetic diversity H（ISSR, 0.107-0.199; SRAP, 0.100-0.153 ）, and Shannon＇s information index I （IS SR, 0.157-0.291; SRAP, 0.148-0.219） indicated a fair amount of genetic variability among the nine populations. Moreover, the high degree of gene differentiation Gst （ISSR, 0.654; SRAP, 0.718） and low gene flow Nm （ISSR, 0.265; SRAP, 0.196） implied that there was significant among-population differentiation, possibly as a result of habitat fragmentation. The matrices of genetic distances and fixation indices （Fst） among the populations correlated well with their geographical distribution （Mantel testR=0.541 5, 0.541 8; P=0.005 0, 0.002 0 andR=0.728 6, 0.641 2; P=-0.001 0, 0.001 0, respectively）; the Rongcheng population in the Shandong peninsula was the only exception. Overall, the genetic differentiation agreed with the geographic isolation. The fair amount of genetic diversity that was revealed in the S. horneri populations in China indicated that the seaweed resources had not been seriously affected by external factors.

关 键 词：Sargassum horneri population genetics ISSR SRAP MARKERS 

分 类 号：S917.3[农业科学—水产科学]