Direct Electrochemistry of Hemoglobin on Vertically Aligned Carbon Hybrid TiO2 Nanotubes and Its Highly Sensitive Biosensor Performance  

作　　者：Meichuan Liu Guohua Zhao Yiting Tang Huijie Shi Nianjun Yang 

机构地区：[1]Department of Chemistry, Tongji University, Shanghai 200092, China [2]Key Laboratory of Yangtze River Water Environment, Ministry of Education, Tongji University, Shanghai 200092, China [3]1m2

出　　处：《Chinese Journal of Chemistry》2013年第2期215-220,共6页中国化学（英文版）

基　　金：the National Natural Science Foundation of China，NSFC Research Fund for International Young Scientists，the Fundamental Research Funds for the Central Universities

摘　　要：The present work is focused on developing a novel biomaterial platform to achieve enhanced direct electron transfer （DET） of hemoprotein and higher biosensor performance on vertically aligned carbon hybrid TiO2 nanotubes （C-TiO2 NTs）. Using a simple surfactant-assisted method, controllable hybridization of TiO2 NTs with conductive amorphous carbon species is realized. The obtained C-TiO2 NTs is ingeniously chosen to serve as an ideal ＂vessel＂ for protein immobilization and biosensor applications. Results show that the appropriate hybridization of C into TiO2 NTs leads to a much better conductivity of TiO2 NTs without destroying their preponderant tubular structures or damaging their excellent biocompatibility and hydrophilicity. When used in loading proteins, the C-TiO2 NTs can be used as a super vessel for rapid and substantive immobilization of hemoglobin （Hb）, with a large surface electroactive Hb coverage （I＊） of 3.3 × 10 9 mol·cm^-2. Enhanced DET of Hb is commendably observed on the constructed Hb/C-TiO2 NTs biosensor with a couple of well-defined redox peaks in a fast electron transfer process. The biosensor further exhibits fast response, high sensitivity and stability for the amperometric biosensing of H202 with the detection limit as low as 3.1 × 10^-8 mol/L.The present work is focused on developing a novel biomaterial platform to achieve enhanced direct electron transfer （DET） of hemoprotein and higher biosensor performance on vertically aligned carbon hybrid TiO2 nanotubes （C-TiO2 NTs）. Using a simple surfactant-assisted method, controllable hybridization of TiO2 NTs with conductive amorphous carbon species is realized. The obtained C-TiO2 NTs is ingeniously chosen to serve as an ideal ＂vessel＂ for protein immobilization and biosensor applications. Results show that the appropriate hybridization of C into TiO2 NTs leads to a much better conductivity of TiO2 NTs without destroying their preponderant tubular structures or damaging their excellent biocompatibility and hydrophilicity. When used in loading proteins, the C-TiO2 NTs can be used as a super vessel for rapid and substantive immobilization of hemoglobin （Hb）, with a large surface electroactive Hb coverage （I＊） of 3.3 × 10 9 mol·cm^-2. Enhanced DET of Hb is commendably observed on the constructed Hb/C-TiO2 NTs biosensor with a couple of well-defined redox peaks in a fast electron transfer process. The biosensor further exhibits fast response, high sensitivity and stability for the amperometric biosensing of H202 with the detection limit as low as 3.1 × 10^-8 mol/L.

关 键 词：carbon hybrid TiO2 nanotubes direct electron transfer electrochemical biosensors HEMOGLOBIN 

分 类 号：TQ423[化学工程] O614.411[理学—无机化学]