PCR-DGGE法分析湛江东海岛海水入侵引起的土壤微生物群落结构变化  

VARIATION IN SOIL MICROBIAL COMMUNITY STRUCTURE INDUCED BY SEAWATER INTRUSION IN EAST ISLAND OF ZHANGJIANG CITY, GUANGDONG PROVINCE,CHINA: A PCR-DGGE STUDY

作　　者：苏洁[1] 王金生[1,2] 戴宁[2] 滕彦国[1] 左锐[2] 

机构地区：[1]北京师范大学水科学研究院,北京100875 [2]地下水污染控制与修复教育部工程研究中心,北京100875

出　　处：《北京师范大学学报（自然科学版）》2013年第2期312-317,共6页Journal of Beijing Normal University(Natural Science)

基　　金：国家自然科学基金资助项目(40773055)

摘　　要：对湛江东海岛研究区水质调查结果显示该地区存在海水入侵现象,为了揭示海水入侵对土壤环境微生物的影响,本文采用PCR-DGGE技术对20个采样点的土壤样品进行微生物群落结构的分析.结果表明:海水入侵对微生物群落结构产生影响,随着远离海岸线微生物种群数量增加,且同一地点不同采样深度微生物群落的组成也存在着差异.根据Shannon-Weaver指数及聚类分析可知,强海水入侵区的多样性指数要低于弱入侵区,海水入侵区近海岸与远海岸的微生物种群相似性有较大差别.The East Island of Zhanjiang City is facing seawater intrusion according to local water quality survey. To investigate effects of seawater intrusion on soil microbes, microbial community structure of 20 soil samples was analyzed using PCR-DGGE. Compared to the results of dominant DGGE bands for Shannon-Weaver and cluster analysis, the extent of seawater intrusion directly influenced soil microbial community structure, microorganism increased with increasing distance from coast. The Shannon-Weaver of strong seawater intrusion area was lower than weak intrusion area; microbial population was different between areas near and far from coasts.

关 键 词：海水入侵 微生物群落 PCR-DGGE 

分 类 号：X53[环境科学与工程—环境工程]