3种非生物诱导剂对红地球葡萄离体叶片中主要茋类物质的诱导作用  

The effects of three non-biotic inducers on major stilbenes contents in detached grape leaves

作　　者：黄方爱[1] 张波[1] 杨晓燕[1] 颜欢[1] 陈韩英[1] 郑秋生[2] 

机构地区：[1]石河子大学药学院,新疆石河子832002 [2]石河子大学新疆特种资源植物药教育部重点实验室,新疆石河子832002

出　　处：《农垦医学》2012年第5期393-396,共4页Journal of Nongken Medicine

基　　金：国家自然科学基金项目(30860032;31160058);新疆生产建设兵团博士资金项目(2011BB018)

摘　　要：茋类物质是葡萄材料中一大类具有特殊药理活性的天然产物,实际操作中由于葡萄叶片含量过低而导致利用率不足。已有报道UV、氯化铝、过氧化氢等非生物诱导剂能够引起一些植物材料茋类物质的积累的同时又能引起材料氧化胁迫。为了研究这3种诱导剂在葡萄离体叶片上的诱导特性及与氧化胁迫的关系,本文首次使用上述3种诱导剂对红地球葡萄离体叶片中4种主要茋类物质含量变化进行分析,并研究过氧化氢酶(CAT)及N-乙酰-L-半胱氨(N-acetyl-L-cysteine)预处理后茋类物质变化规律。诱导剂处理后,白藜芦醇、白藜芦醇苷、紫檀茋、ε-viniferin等茋类物质都有不同程度的升高,其中紫外照射处理10min,暗室放置24h对白藜芦醇的诱导效果最为明显,鲜重达到200μg/g。加完抑制剂CAT和NAC后,茋类物质变化不规律,其中H2O2诱导加抑制剂CAT对白藜芦醇的抑制效果最佳,含量从113μg/g降低到16.5μg/g。结果说明,3种诱导剂对红地球葡萄离体叶片茋类物质类别及其含量具有特殊的影响作用,且此与材料内部氧胁迫水平存在一定联系。The content of the stilbenes were lower in normal grape leaves,so it is difficult as the use of medicinal ex- tract. The abiotic inducers （H202, UV,AIC13 ） previously reported to elevated the contents of stilbenes in various materials with oxidative stress simultaneously. In this study, aims to study the relationships between 4 stilbenes accumulation and oxi- dative stress, grape leaves were induced by the abiotic inducers and then processed by the corresponding inhibitors （N-ace- tyl-L-cysteine）. The content changes of resveratrol, polydatin, pterostilbene and ε-viniferin were analyzed by HPLC. The re- suits showed that, after the treatment of the inducers, the stilbene had different degrees, of which the effect of UV-induced is the most obvious,the maximum content of resveratrol 200μg/g in treated leaves. Alter the inhibitors（ CAT, NAC）weread- ded. the content changes of the stilbenes were not in the law, inhibitors of H2 02-induced and CAT on the inhibitory effect of resveratrol is best,the content of resveratroJfrom ll3μg/g reduced to 16.5μg/g. The result showed that,Three kinds of in- ducers have the special influence function to the detached grape leaves （Vitis vinifera cv. Red Globe）of category and the content, also material interior oxygen coercion level existence certain relation.

关 键 词：诱导剂 白藜芦醇 茋类物质 葡萄 离体叶片 

分 类 号：R284.2[医药卫生—中药学]