黄芪多糖对多发性骨髓瘤患者骨髓间充质干细胞增殖与细胞因子表达的影响  

Study on the Effect of APS on the Proliferation and Cytokines Secretion of the Bone Mesenchymal Stem Cells Drived from Patient with Multiple Myeloma

作　　者：刘琳[1] 白海[2] 王存邦[2] 潘耀柱[2] 葸瑞[2] 魏玉萍[3] 

机构地区：[1]甘肃中医学院,甘肃兰州730050 [2]兰州军区兰州总医院全军血液病中心,甘肃兰州730050 [3]宁夏回族自治区人民医院血液科,宁夏银川750012

出　　处：《现代生物医学进展》2013年第10期1873-1877,共5页Progress in Modern Biomedicine

基　　金：甘肃省科技重大专项(1102FKDA005)

摘　　要：目的:探讨黄芪多糖对多发性骨髓瘤(multiple myeloma,MM)患者骨髓间充质干细胞(bone mesenchymal stem cells,BMSC)增殖与细胞因子表达的影响。方法:采用密度梯度离心法分离MM患者骨髓间充质干细胞(MM-BMSC)及正常人骨髓间充质干细胞(ND-BMSC)。取第3代BMSC,测定细胞的生长曲线及细胞因子的表达。分别采用MTT法、细胞流式术检测不同浓度APS对MM-BMSC的细胞增殖、细胞周期的影响,ELISA法测定IL-1β与IL-6的表达水平。结果:成功分离得到MM-BMSC和ND-BM-SC,细胞生长曲线显示MM-BMSC增殖缓慢,倍增时间为86 h,而ND-BMSC的倍增时间为60 h,两者相比差异具有统计学差异(P<0.05)。基础正常情况下,MM-BMSC与ND-MSC分泌的IL-1β、IL-6的水平有明显差异(P<0.05)。APS可促进MM-BMSC增殖,其中1 mg/mL APS的作用最为明显(P<0.01)。MM-BMSC基本都处于静止期,1 mg/mL APS处理的MM-BMSC处于G0/G1期细胞比例较其明显降低(P<0.05),S,G2/M期细胞比例较其明显增高(P<0.05),且1 mg/mL APS可显著下调MM-BMSC分泌IL-1β及IL-6的水平(P<0.05)。结论:多发性骨髓瘤患者骨髓间充质干细胞IL-1β、IL-6分泌水平异常,1 mg/mL黄芪多糖可促进骨髓间充质干细胞的增殖并下调其IL-1β,IL-6的分泌。Objective： To explore the effect of astragalus polysaccharide（APS） on the proliferation and the secretion of IL-1β,IL-6 of mesenchymal stem cell（MSC） in patient with multiple myeloma（MM） in vitro.Methods： MSC of MM patient and normal donator were isolated from bone marrow by isodensity centrifugation.Selected BMSC of 3rd,the proliferation time and cytokine secretion of bone marrow mesenchymal stem cell of multiple myeloma（MM-BMSC） were detected compared with bone marrow mesenchymal stem cell of normal donator（ND-BMSC）.Meanwhile the promoting proliferation effect of APS on MM-BMSC were detected by MTT and the effect of APS on cell cycle of MM-BMSC were investigate,ELISA was performed to measure the concentrations of IL-1β and IL-6 of MM-BMSC treated with different concentration of APS.Results： MM-BMSC and ND-BMSC were isolated successfully,the proliferation time of MM-BMSC longer than that of ND-BMSC（86h vs 60h,P0.05） were performed by growth curve.The concentration of IL-1β and IL-6 of MM-BMSC had significant abnormal secretion when it compared with ND-BMSC（P0.05）.APS could obviously increase the proliferation of MM-BMSC,especially APS on 1mg/ml had the most effect（P0.01）.Flow cytometer（FCM） detected cell cycle display,MM-BMSC basic were in resting stage,APS on 1mg/ml significantly reduced the action of cells in G0/G1（P0.05） phase cell proportion,and S,G2/M phase cell were higher than self-control group（P0.05）.Compared with the control group,APS down regulated the secretion of IL-1β and IL-6 of MM-BMSC（P0.05）.Conclusions： The expression of IL-1β and IL-6 of BMSC of MM existed abnormally,and APS could increase the proliferation of BMSC and induced the secretion of IL-1β and IL-6.

关 键 词：骨髓基质细胞 多发性骨髓瘤 IL-1Β IL-6 黄芪多糖 

分 类 号：Q25[生物学—细胞生物学] R285.5[医药卫生—中药学]