丙泊酚对过氧化氢诱导人红细胞氧化应激状态下一氧化氮通路的影响  

Effects of Propofol on the Erythrocytes Nitric Oxide Pathway under Oxidative Stress Induced by Hydrogen Peroxide in Vitro

作　　者：魏国[1] 叶博[1] 范勤[1] 张国荣[1] 

机构地区：[1]安徽医科大学空军临床学院,北京100142

出　　处：《现代生物医学进展》2013年第12期2249-2252,2288,共5页Progress in Modern Biomedicine

基　　金：全军"十二五"基金项目(CKJ11J020)

摘　　要：目的:一氧化氮(nitric oxide,NO)作为体内的一种细胞信使分子,在心血管活动中起重要作用。NO水平及其在体内的合成代谢通路与临床麻醉、危重症、术后恢复等密切相关。本实验通过使用H2O2在体外诱导人红细胞氧化应激反应,观察红细胞NO,eNOS,NO3-和NO2-水平的变化,及丙泊酚对这一变化的影响。方法:健康成年人红细胞制成2%红细胞悬液,分为10组:对照组(C组)、H2O2组(H组)、丙泊酚12.5μmol/L组,丙泊酚25μmol/L组,丙泊酚50μmol/L组,丙泊酚100μmol/L组,丙泊酚12.5μmol/L+H2O2组(P12.5+H组)、丙泊酚25μmol/L+H2O2组(P25+H组)、丙泊酚50μmol/L+H2O2组(P50+H组),丙泊酚100μmol/L+H2O2组(P100+H组)。各组H2O2的反应浓度均为200μmol/L,孵育30 min,测定红细胞NO,eNOS,NO3-和NO2-水平的变化。结果:P50组(4.97±0.58)NO水平高于其余各组(P<0.05);H组(4.96±0.52)NO水平高于其它组(P<0.05);与C组(1.34±0.29)相比,P50组(2.23±0.33)和H组(2.33±0.39)eNOS水平升高(P<0.05);NO3-水平H组(43.78±2.13)比C组(52.06±2.14)低(P=0.017);NO2-水平H组(13.32±2.04)比C组(34.14±1.48)低(P=0.025)。结论:丙泊酚和H2O2能够使红细胞NO和eNOS水平的升高;H2O2引起红细胞NO升高和NO3-,NO2-的降低。我们推断,H2O2使体内产生的过量NO,会对机体产生害影响,而丙泊酚通过清除自由基、抑制H2O2诱导的红细胞硝酸盐-亚硝酸盐-一氧化氮通路向氧化相移动来维持NO水平,实现维持NO生物利用率及保护人红细胞抵抗氧化损伤。Objective： As a cellular messenger molecules, Nitric oxide plays an important role in cardiovascular activity. The level of NO and its metabolites are closely related to clinical anesthesia, critical care and postoperative recovery. To explore the effects of propofol on erythrocyte stimulated by H2O2, which was analyzed by NO, eNOS, NO3- and NO2- of erythrocyte, in vitro. Methods： Human erythrocytes isolated from healthy volunteers were made to 2% suspension and divided into 10 groups： group C （ringer solution alone）, group H （HE0： alone）, group P12.5（12.5μmol/L ofpropofo）, group P25（25 μmol/L ofpropofo）, group P50（50μmol/L ofpropofo）, group P100 （100 μmol/L ofpropofo）, group P12.5＋H （12.5μmol/L ofpropofol and H2O2）, group P25＋H （25 μmol/L ofpropofol and H2O2）, group P50＋H （50μmol/L ofpropofol and H2O2） and group P100＋H （100μmol/L ofpropofol and H2O2. H2O2was used at a concentration of 200 μmol/L. Both erythrocyte NO and eNOS were determined by the method of Griess. Results： The levels of NO at group P50 （4.97±0.58） and group H （4.96±0.52） is higher than the other groups （P〈0.05）; Compared with the group C （1.34±0.29）, the levels of eNOS at group P50 （2.23±0.33） and group H （2.33±0.39） were increased （P 〈0.05）; The level of NO3- at group H （43.78±2.13） was lower than group C （52.06±2.14） （P = 0.017）; The level of NO2- group H （13.32±2.04） was lower than group C （34.14 ＋ 1.48） （P = 0.025）. Conclusions： Propofol and H202 can elevate the levels of NO and eNOS in erythrocyte; HEO2-induced increase the level of NO and the reduction of NO3- and NO3- in erythrocyte, which was consistent with the alternative pathway of NO. We conclude that propofol can maintain the level of NO by scavenging free radicals, maintaining NO bioavailability and protecting human erythrocytes against oxidative damage.

关 键 词：红细胞 丙泊酚 一氧化氮 一氧化氮合酶 过氧化氢 

分 类 号：R971.2[医药卫生—药品] Q813[医药卫生—药学]