白介素18启动子区基因多态性与儿童EV71感染相关性研究(英文)  

Association of Interleukin-18 Gene Promoter Polymorphism with the Susceptibility to Enterovirus 71 Infection in Children

作　　者：韩伟平[1] 陈宗波[1] 韩振靓[1] 李继安[1] 吕铁刚[1] 刘培培 胡静飞 辛丹丹[1] 

机构地区：[1]青岛大学医学院附属医院,山东青岛266003 [2]青岛妇女儿童医院,山东青岛266003

出　　处：《现代生物医学进展》2013年第13期2445-2449,共5页Progress in Modern Biomedicine

基　　金：National Nature Science Support Projects(31171212)~~

摘　　要：目的:研究白介素18基因启动子多态性与儿童EV71感染遗传易感性的关系。方法:收集EV71感染患儿177例,单纯HFMD组127例,HFMD并脑炎组50例,提取外周血DNA,用序列特异性引物-聚合酶链反应(SSP-PCR)技术及基因测序法检测IL-18启动子区-137G/C、-607A/C位点的基因多态性。结果:EV71感染患儿与健康儿童IL-18-607基因型以CA为主,AA、CC次之,EV71感染患儿AA基因型、A等位基因分布频率显著高于健康儿童。EV71感染HFMD并脑炎组患儿AA基因型分布显著高于单纯HFMD组患儿,差异有统计学意义。EV71感染患儿与健康儿童IL-18-137基因型以CC为主,CG次之,GG占少数。该位点基因型及等位基因在EV71感染组与健康儿童、单纯HFMD与HFMD并脑炎组的分布无显著差异。结论:IL-18基因多态性与EV71感染相关,IL-18-607AA基因型、A等位基因携带儿童更易感染EV71病毒,且AA基因型患儿易并发脑炎,-607AA基因型可能为EV71感染的易感基因型。-137C/G位点基因多态性与EV71感染无相关性。Objective： To investigate the relationship between interleukin-18 gene promoter polymorphism and the susceptibility of enterovirus 71 infection in Chinese children.Methods： Using the method of the polymerase chain reaction with specific primers（SSP-PCR） and sequencing technique.The two single-nucleotide polymorphisms of IL-18 gene promoter,-137 G/C and-607 C/A,in 50 EV71 encephalitis patients,127 hand,foot and mouth disease patients without complications and 226 age and sex matched controls were detected.Results： The CA genotype frequency of IL-18-607C/A in EV71 infection patients and healthy children was the higher,followed by AA genotype and CC genotype.The AA genotype and the A allele in IL-18-607C/A had a higher frequency in the EV71 infection patients compared that in the controls.Similarly,in the encephalitis group,the AA genotype frequency was higher than that in the HFMD patients without complications.The IL-18-137CC genotype frequency was highest in the EV71 infection children and controls,followed by CG genotype,and the frequency of GG genotype was lowest.The frequency of all genotypes and alleles in IL-18-137C/G had no significant difference between EV71 infection patients and controls,or between encephalitis patients and HFMD patients without complications.Conclusion： The date suggested that Interleukin-18 gene promoter polymorphism had relationship with EV71 infection.The AA genotype and A allele in IL-18-607A/C may increased the incidence of enterovirus 71 infection in children.Moreover,the carriers of AA genotype were more susceptible to EV71 encephalitis than that in other genotypes.There was no evident association between G/C polymorphism in IL-18-137 gene and EV71 infection.All these can further understand the genetic mechanisms of EV71 infection.

关 键 词：肠道病毒71型 白介素18 基因多态性 

分 类 号：R725.1[医药卫生—儿科]