孕中期巨细胞病毒宫内感染与血Free-β-HCG水平的相关性研究  

The Correlation between Midpregnancy Intrauterine Infection of Cytomegalovirus and Blood Free-β-HCG Levels

作　　者：张立群[1] 钱东萌[1] 刘海婷[2] 巩振华[2] 王斌[1] 

机构地区：[1]青岛大学微生物学教研室,山东青岛266071 [2]淄博市妇幼保健院遗传实验室,山东淄博255029

出　　处：《现代生物医学进展》2013年第13期2536-2538,2542,共4页Progress in Modern Biomedicine

摘　　要：目的:通过检测孕中期妇女人巨细胞病毒(HCMV)活动性感染、宫内感染以及血Free-β-HCG水平,分析血Free-β-HCG水平与HCMV宫内感染的相关性,初步探讨其可能影响机制。方法:通过酶免法测孕妇血HCMV-IgM和定荧光定量PCR对孕中期妇女血清和羊水中HCMV-DNA的检测以及DILFIA法(时间分辨免疫荧光法)定量检测孕中期妊娠妇女血Free-β-HCG的含量,研究HCMV感染状况与Free-β-HCG之间的相关性,探讨HCMV对母血Free-β-HCG水平的影响。结果:718例样本中共检出HCMV-IgM阳性和(或)HCMV-DNA阳性者共43例并进一步行产前诊断羊水HCMV-DNA阳性14例。孕中期HCMV活动性感染率为5.98%,HCMV宫内感染率为2.22%。孕中期感染组Free-β-HCG含量:7.32±2.25 ng/ml,非感染组:8.47±3.17 ng/ml,对照组:10.10±3.67 ng/ml。结论:HCMV宫内感染组比非感染组孕中期外周血Free-β-hcG的测定水平降低,两者结果之间的差异具有统计学意义。HCMV可通过损伤胎盘绒毛滋养层细胞,使Free-β-hcG的分泌减少。Objective： Through the detection the second trimester women cytomegalovirus（HCMV） active infection,intrauterine infection and blood Free-β-HCG levels,to analyse the correlation between blood Free-β-HCG levels and HCMV intrauterine infection and explore its possible influencing mechanism.Methods： ELISA and PCR technique were used to detect specific hCMV IgM antibodies and HCMV DNA in blood of midpregnant women to diagnose materal CMV intrauterine infection;And DILFIA for quantitative detection of blood Free-β-HCG content.To research the influence of HCMV intrauterine to the mother blood Free-β-HCG levels.Results： The positive rate of 718 samples was 2.22 %.The serum leval of the Free-β-HCG of the groups was 7.32±2.25 ng/ml,8.47±3.17 ng/ml,10.10±3.67 ng/ml respectively.Conclusions： Free-β-hcG level of the HCMV infection group was lower than the noninfenction group fo the midpregnancy women,there were significant differences between the two results.HCMV can damage placental villous trophoblast cells to decrease the secretion of the Free-β-hcG.

关 键 词：人巨细胞病毒 宫内感染 妊娠中期 血Free-β-HCG 

分 类 号：R714.62[医药卫生—妇产科学]