马齿苋多糖的乙醇分级纯化及抗氧化研究  

Ethanol Fractional Precipitation and Antioxidation Character of Polysaccharides of Portulaca oleracea L.

作　　者：姚秋萍[1] 姚芝荣[1] 

机构地区：[1]贵州民族大学化学与环境科学学院,贵州贵阳550025

出　　处：《安徽农业科学》2013年第8期3656-3658,共3页Journal of Anhui Agricultural Sciences

基　　金：贵州省科技厅项目(黔科合J字[2010]2263号);贵州民族大学引进人才科研项目

摘　　要：[目的]研究马齿苋各级分多糖的抗氧化作用。[方法]以热水浸提马齿苋,采用35%、55%、75%和95%的乙醇分级醇沉马齿苋多糖提取物,得马齿苋各级分多糖POP1、POP2、POP3和POP4,分析其对羟基自由基和超氧阴离子自由基的清除作用。[结果]醇沉马齿苋多糖量的比例为POP1∶POP2∶POP3∶POP4为0.83∶1.25∶1.82∶2.16;各级分多糖抗氧化能力随多糖浓度的上升而增强,呈现良好的量效关系,并且在等浓度条件下其抗氧化能力也表现出明显的差异,清除能力为POP4>POP3>POP2>POP1。[结论]乙醇浓度95%醇沉的马齿苋多糖具有较强的抗氧化作用。[Objective] To study the antioxidant activity of polysaccharides of Portulaca oleracea.(POP).[Method] A polysaccharide-rich extract was obtained from P.oleracea by hot water extraction.The experiment models of amount of polysaccharide precipitated by different concentrations of ethanol(35%、55%、75% and 95%) include system and system.[Result] 35%ethanol,55%ethanol,75%ethanol and 95%ethanol's precipitation amount POP are POP1∶POP2∶POP3∶POP4=0.83∶1.25∶1.82∶2.16;the higher the concentration of polysaccharide,the stronger removing ability of hydroxyl radicals and superoxide anion,precipitation of the polysaccharide concentration in conditions of antioxidant activity was: POP4>POP3>POP2>POP1.[Conclusion] 95% ethanol's precipitation polysaccharide of P.oleracea shows a significant antioxidant activity.

关 键 词：马齿苋多糖 乙醇浓度 抗氧化 

分 类 号：S567[农业科学—中草药栽培] TS245[农业科学—作物学]