Establishment of Experimental Mastitis Model by Lipopolysaccharide via Teat Duct in Rabbit  

作　　者：CAO Sui-zhong YANG De-ying YAO Xue-ping YU Shu-min SHEN Liu-hong LIU Chang-song 

机构地区：[1]College of Veterinary Medicine,Sichuan Agricultural University

出　　处：《Animal Husbandry and Feed Science》2013年第2期62-64,共3页动物与饲料科学（英文版）

基　　金：supported by China Postdoctoral Science Foundation(20090451250);the Program for Changjiang Scholars and Innovative Research Team in Ministry of Education of China(IRT0848);the Project of Youth Fund of Education of Department of Sichuan Province(09ZB054)

摘　　要：[ Objective] This study was conducted to explore the method of establishing Lipopolysaccharide （LPS） -induced mastitis pathological models and reveal dynamic pathological changes of mammary tissue before and after LPS perfusion, finally providing convenient animal models for establishing LPS- induced acute clinical mastitis researches. [ Method] Twelve lactating rabbits （the 7th day after parturition） were perfused with LPS into the fourth mammary gland via the teat duct. Exactly at 2h before perfusion, 6 h, 12 h, 24 h, 48 h, 72 h, 5 d and 7 d after, the indexes such as rectal temperature, total white blood calls and neutrophils, C -response protein （CPR） content and lactate dehydrogenase （LDH） activity were determined, respectively. Then the rabbits were euthanatized and the mammary glands were removed and fixed for histopathologic evalua- tions. [Result] The results showed that inflammatory cells were observed in mammary tissue 6 h after LPS perfusion, structure of mammary tissue disorderly at 24 h, self - regeneration after 48 h and back to normal at 7 d. LDH activity was increasing significantly （ P 〈 0.05） at 12 h （ P 〈 0.05）, peaking at 24 h, decreasing at 5 d （ P 〈 0.05） and returning to health at 7 d. CRP content was increasing significantly （ P 〈 0.05） at 6 to 72 h, pea- king at 12 h, decreasing at 48 h, back to normal at 5 d. [ Conclusion] The results suggested that the rabbit experimental mastitis model was suc- cassfullv constructed bv LPS Derfusion via teat duct.[ Objective] This study was conducted to explore the method of establishing Lipopolysaccharide （LPS） -induced mastitis pathological models and reveal dynamic pathological changes of mammary tissue before and after LPS perfusion, finally providing convenient animal models for establishing LPS- induced acute clinical mastitis researches. [ Method] Twelve lactating rabbits （the 7th day after parturition） were perfused with LPS into the fourth mammary gland via the teat duct. Exactly at 2h before perfusion, 6 h, 12 h, 24 h, 48 h, 72 h, 5 d and 7 d after, the indexes such as rectal temperature, total white blood calls and neutrophils, C -response protein （CPR） content and lactate dehydrogenase （LDH） activity were determined, respectively. Then the rabbits were euthanatized and the mammary glands were removed and fixed for histopathologic evalua- tions. [Result] The results showed that inflammatory cells were observed in mammary tissue 6 h after LPS perfusion, structure of mammary tissue disorderly at 24 h, self - regeneration after 48 h and back to normal at 7 d. LDH activity was increasing significantly （ P 〈 0.05） at 12 h （ P 〈 0.05）, peaking at 24 h, decreasing at 5 d （ P 〈 0.05） and returning to health at 7 d. CRP content was increasing significantly （ P 〈 0.05） at 6 to 72 h, pea- king at 12 h, decreasing at 48 h, back to normal at 5 d. [ Conclusion] The results suggested that the rabbit experimental mastitis model was suc- cassfullv constructed bv LPS Derfusion via teat duct.

关 键 词：Upopolysaccharide（LPS） Animal model of mastitis RABBIT C-response protein （CRP） 

分 类 号：S857.26[农业科学—临床兽医学]