水环境中多种磺胺类抗生素残留的HPLC同步检测  

Simultaneous Determination of Sulfonamides Residues in Water Environment by HPLC

作　　者：李清雪[1] 刘巨波[1] 刘娟[1] 

机构地区：[1]河北工程大学城市建设学院,河北邯郸056038

出　　处：《河北师范大学学报（自然科学版）》2013年第3期270-275,共6页Journal of Hebei Normal University：Natural Science

基　　金：邯郸市科技攻关项目(0823109075-2)

摘　　要：建立了固相萃取-高效液相色谱法(SPE-HPLC)同步检测水环境中9种磺胺类抗生素残留的分析方法.选用Oasis HLB固相萃取小柱,研究不同pH值、洗脱溶剂、洗脱体积对萃取效果的影响;乙腈和0.1%(体积分数)甲酸水溶液作为流动相,采用梯度洗脱方法,检测波长268nm.结果表明,9种磺胺类抗生素的仪器检出限为64.9～300μg/L,仪器的稳定性和准确度均较好,在纯水、自来水、污水及污水处理厂出水中磺胺类抗生素的回收率分别为13.6%～93.7%,22.4%～87.4%,未检出～60.1%和未检出～63.9%,除磺胺脒外,回收率基本都大于50%.An analytical method to simultaneously determinate nine kinds of sulfonamide residues in water environment by SPE-HPLC was established. We selected the Oasis HLB solid phase extraction col- umn to research the effects of different pH values,elution solvents,and elution volume on extraction abili- ties. The mobile phase was composed of acetronitrile and 0. 1% formic acid （gradient elution） with a detec- tion wavelength of 268 nm. The results indicated that the instrument detection limit of nine kinds of sulfon- amides from 64.9 to 300μg/L,which revealed the stability and accuracy of the instrument. The recoveries of sulfonamides from pure water,tap water,sewage and sewage effluents were 13.6 %-93.7 %,22.4 %- 87.4 % ,n. d. -60.1 % and n. d. -63.9 % （n. d. =not detected） respectively. All the recoveries were grea- ter than 50% except sulphaguanidine.

关 键 词：固相萃取 高效液相色谱 磺胺类抗生素 污水处理 

分 类 号：O657.72[理学—分析化学]