鼠疟原虫引起血小板减少药物干预效果的观察  

Drug intervention effects on thrombocytopenia due to Plasmodium berghei infection in mice

作　　者：区德锦[1] 韦海艳[2] 邹春燕[3] 崔立旺[4] 黄亚铭[2] 

机构地区：[1]广州医学院第三附属医院检验科,广东广州510150 [2]广西壮族自治区疾病预防控制中心原虫病科,广西南宁530028 [3]广西医科大学甚而医学院寄生虫教研室,广西南宁530021 [4]宾夕法尼亚州立大学昆虫学系

出　　处：《中国热带医学》2013年第2期131-133,共3页China Tropical Medicine

基　　金：国家自然科学基金项目(No.30660169);广西自然科学基金项目(No.0991013.08-42-01A)

摘　　要：目的了解退烧药、抗生素及激素对感染鼠疟原虫引起的血小板减少是否具有恢复的效果。方法健康昆明小鼠腹腔接种感染伯氏鼠疟原虫,当感染鼠血小板明显低于正常值后,分别采用临床常用的大环内酯类、β内酰胺类和喹诺酮类抗生素、退烧药及激素类药物按照人体治疗量的10倍灌服或肌注方法给药3d,每12h采血1次做血小板计数观察。每5只小鼠为一个观察试验组,每种药物采用一组小鼠试验观察取平均数据,并设正常对照组。结果正常鼠血小板平均计数为256×109/L。健康鼠感染鼠疟原虫后第8d,各组感染鼠血小板计数平均降至90×109/L后,开始灌服各类抗生素和肌注氨基比林及地塞米松,除阿奇霉素观察组治疗3d后血小板计数平均恢复至201×109/L水平外,其它抗生素和退烧药及激素均对感染鼠疟原虫所引起的血小板明显减少恢复无影响。结论除阿奇霉素外,复方氨基比林、阿莫西林、左氧氟沙星、地塞米松对鼠疟原虫感染小鼠血小板减少无明显效果。Objective To analyze the common antipyretics,antibiotics and corticosteroids on thrombocytopenia due to Plasmodium berghei infection in mice. Methods Healthy Kunming mice were intraperitoneally inoculated with Plasmodium berghei and treated with β -lactams,quinolones,antipyretics and corticosteroids at dose of 10 times that of human dose for 3 days by irrigation or intramuscular injection when platelet count markedly below normal value.Then blood samples were obtained for every 12 hours and platelet count was recorded. Observation group consisted of five mice and each drug was tested in a group and with control group. Results The normal mice platelet count averaged for 256 x 109/L and the platelet count dropped to an average count of 90 x 109/L in each group 8 days after infeetion with Plasmodium berghei. The platelet count returned to 201 x 109/L in azithromycin-treated group only 3 days after treated with various antibiotics and muscular injection of aminophenazone and dexamethasone,and other antibiotics, antipyretic and corticosteroids showed no effect on thrombocytopenia recovery in mice infected with Plasmodium berghei. Conclusions Except azithromycin,aminophenazone,amoxiciUin,levofloxacin and dexamethasone showed on effects on platelet recovery in mice infected with Plasmodium berghei

关 键 词：鼠疟原虫 血小板计数 抗生素 MICE 

分 类 号：R531.3[医药卫生—内科学]