盐胁迫条件下蒲公英叶片蛋白的双向电泳及其图谱分析  

2-DE Method and proteomic pattern analysis in Taraxacum mongolicum leaves under salt stress

作　　者：冯昕[1] 孙浩[1] 王吉中[1] 何培新[1] 

机构地区：[1]郑州轻工业学院食品与生物工程学院,郑州450002

出　　处：《食品科技》2013年第5期108-111,共4页Food Science and Technology

基　　金：中国科学院知识创新工程重要方向项目(KSCXZ-YW-N-46-02-04)

摘　　要：目的:研究盐胁迫条件下蒲公英叶片中蛋白表达的动态变化规律,探讨蒲公英耐盐的生化机理。方法:对其正常和胁迫条件下的蒲公英叶片进行蛋白双向电泳,通过ImageMaster2-DE分析系统对结果进行定性、定量分析。结果:胁迫条件下蒲公英叶片的2-DE图谱共检测到457个蛋白点,共有12个蛋白点在表达的质和量上有明显变化,其中3个蛋白点在胁迫条件下2-DE图谱中表达量明显高于正常对照的蛋白点,9个蛋白点表达量明显低于正常对照的蛋白点,在胁迫条件下2-DE图谱中新增2个蛋白点,缺失1个蛋白点。结论:差异表达的蛋白点可能就是耐盐性相关的蛋白质。To compare the global protein maps of Taraxacum mongolicum between normal leaves and leaves under salt stress by means of two-dimensional electrophoresis （2-DE） so as to explore the biochemical mechanisms of salt tolerance of Taraxacum mongolicum. The whole leaf protein- was prepared by improved TCA-acetone precipitation method and then separated by 2D-PAGE. The electrophoregrams of Taraxacum mongolicum proteome between normal leaves and salt-stress leaves were evaluated qualitatively and quantitatively with the Image Master 2-DE analyzer. The results showed that total 457 protein spots were detected in 2-DE and the qualitative and quantitative changes in 12 protein expression of salt-stressed versus control leaves were detected, three of which were significantly up-regulated and nine significantly down-regulated in Salt-stressed leaves. Furthermore, two new protein spots emerged and one spot missed in electrophoregrams of salt-stressed leaves. The differential displayed protein spots might relate to the salt tolerance.

关 键 词：蒲公英 盐胁迫 双向电泳 图谱分析 

分 类 号：Q949.95[生物学—植物学]