UPLC-UV法快速测定烟草中游离茄尼醇的含量  

Fast Determination of Free Solanesol in Tobacco with UPLC-UV Spectrophotometer

作　　者：韩敬美 刘春波[1] 赵伟[1] 何沛[1] 刘志华[1] 

机构地区：[1]云南烟草科学研究院云南省烟草化学重点实验室,昆明市高新区科医路61号650106

出　　处：《烟草科技》2013年第5期61-63,71,共4页Tobacco Science & Technology

基　　金：中国烟草总公司科技重大专项项目"卷烟主流烟气中苯并芘的形成机理研究"(110201001001);云南中烟工业公司科技开发计划项目(2012JC02)

摘　　要：为了简化烟草样品的前处理步骤，采用甲醇直接萃取烟草样品得到甲醇萃取液，优化超高效液相色谱的测试条件，测定了甲醇萃取液中的茄尼醇含量。结果表明：①最佳萃取条件为：萃取溶剂为甲醇。萃取温度25℃，萃取时间20min；②茄尼醇工作曲线的浓度范围为10～200mg／L时，质量浓度与峰面积具有良好的线性关系，相关系数为0．9997；③方法的检出限为0．054mg／L，相对标准偏差为2．1％，平均回收率为105．5％。该方法可以快速、简便、准确地测试烟草中游离茄尼醇的含量。In order to simplify the pretreatment procedures, tobacco sample was directly extracted by methanol and the UPLC-UV conditions were optimized, then the solanesol content in the obtained extract was determined. The results showed that： 1） The optimal conditions of extraction were： methanol as solvent and extracting for 20 minutes at 25 ℃. 2） The concentration of working curve of this method ranged from 10 to 200 mg/L. Good linear relationship existed between mass concentrations and peak areas with the correlation coefficient of 0.9997; 3） The detection limit of this method was 0.054 mg/L with the relative standard deviation of 2.1% and the average recoveries of 105.5%. This method is fast, simple, accurate and suitable for the determination of solanesol content in tobacco.

关 键 词：茄尼醇 超高效液相色谱-紫外检测器 烟草 萃取 

分 类 号：TS411.1[农业科学—烟草工业] O657.7[理学—分析化学]