经下丘脑室旁核给予核转录因子κB抑制剂吡咯烷二硫基甲酸盐对高血压大鼠的影响  

NF-KB blockade PDTC via PVN has an effect on hypertensive rat

作　　者：藏好晶[1] 齐杰[2] 许艳艳[1] 于晓静[2] 苏青[2] 康玉明[1] 

机构地区：[1]山西医科大学生理系,太原市030001 [2]西安交通大学生理系,太原市030001

出　　处：《中国心血管病研究》2013年第5期373-376,I0002,共5页Chinese Journal of Cardiovascular Research

基　　金：国家教育部博士点基金（项目编号：20101417110002）;山西省自然科学基金（项目编号：2010011052一1）

摘　　要：目的观察核转录因子KB（NF-kB）拮抗剂对高血压大鼠下丘脑室旁核中血管紧张素Ⅱ受体（AT1-R）水平的影响，探讨脑内NF-kB是否通过调节下丘脑室旁核（PVN）中的AT-R在高血压发病中发挥作用。方法选取体重250-275g雄性SD大鼠，随机分为4组：高血压干预组（ANGⅡ＋PDTC）、高血压对照组（ANGⅡ＋VEH）、假手术干预组（NS＋PDTC）和假手术对照组（NS＋VEH）。记录3d基础血压后，麻醉状态下肩胛区皮下给予ANGⅡ（200ng·kg^-1·min^-1）诱导高血压，对照组给予生理盐水（Ns）。干预组通过下丘脑室旁核插管给予吡咯烷二硫基甲酸盐（PDTC）；对照组给予人工脑脊液（VEH）。术后采用无创血压测量系统监测血压，4周后使用免疫荧光技术检测下丘脑室旁核AT1-R水平。结果ANGⅡ＋VEH与NS＋VEH相比，下丘脑室旁核中AT1-R水平明显升高，血压升高（P〈0．05）；ANGⅡ＋PDTC与ANGⅡ＋VEH相比，下丘脑室旁核中AT1-R水平降低，血压下降（P〈0．05）。结论中枢NF-kB通过调节下丘脑室旁核AT1-R水平参与高血压的发病机制，阻断NF-kB可在一定程度上降低血压值。Objective To investigate the effects of NF-KB blockade in the paraventricular nucleus of hy- pothalamus （PVN） on the level of ATj-R in hypertensive rat, and to explore the possible role of brain NF-KB in hypertensive rats through modulation of AT1-R in paraventricular nucleus （PVN）. Methods Adult male Sprage- Dawley rats（250-275 g） were randomly divided into 4 groups, hypertension treatment group（ANG Ⅱ ＋PDTC ）, hy- pertensiofi control group（ANG Ⅱ ＋VEH）, sham treatment group（NS＋PDTC）, sham control group （NS＋VEH）. Af- ter three days＇ baseline blood pressure recordings, rats were implanted subcutaneously with an angiotensin II 200 ng· kg-l ·min-1 filled osmotic minipump to induce hypertension. Rats in sham group were administered with saline （NS）. PVN cannulae were implanted for the injection of PDTC （5 μg/h） and artificial cerebrospinal fluid （VEH, 0.25 μ1/h）. Immunofluorescence was used to test the level of AT1-R in PVN 4 weeks later. Results Hy- pertensive rats showed a significant increase in the expression of AT1-R in the PVN when compared with SHAM rats （P〈0.05）. Treatment with PDTC was shown to reduce the AT1-R level （P〈0.05）. Conclusion Brain NF- KB involves in hypertension by modulating AT1-R in the PVN, blocking NF-KB can reduce blood pressure.

关 键 词：高血压 下丘脑室旁核 核转录因子KB 血管紧张素Ⅱ 血管紧张素Ⅱ受体 

分 类 号：R544.1[医药卫生—心血管疾病]