缺血后处理对大鼠肺缺血-再灌注损伤中肺表面活性蛋白A及动脉血气的影响  

The effects of ischemic postconditioning on SP-A, arterial blood gas in rat lung during ischemia-reperfusion injury

作　　者：马立民[1] 饶旭光[2] 段明科 杨伟[1] 林国雄[4] 

机构地区：[1]汕头大学医学院第二附属医院胸心外科,广东省515041 [2]广州医学院附属肿瘤医院胸外科,广东省515041 [3]厦门市第二人民医院胸心外科,广东省515041 [4]海南省人民医院小儿外科,广东省515041

出　　处：《中国心血管病研究》2013年第5期380-383,I0003,共5页Chinese Journal of Cardiovascular Research

基　　金：汕头市重点科技计划项目（项目编号：汕府科[2010]63号）

摘　　要：目的研究缺血后处理对于肺缺血一再灌注损伤的保护作用。方法60只雄性sD大鼠随机分为2个模型制作组，每个模型制作组30只随机分为5组：假手术组（S）、缺血一再灌注（I／R）45min组和120min组，以及缺血后处理（IPO）45min组和120min组。用免疫组化法测定肺组织中肺表面活性蛋白A（sP—A）的表达水平，动脉血行动脉血气分析，测定肺组织的通透性（^125Ⅰ标记牛血清白蛋白的漏出量表示），光镜及电镜下观察肺组织的病理形态变化。结果IPO组与I／R组相比较，SP—A表达水平明显升高（P〈O．01），血气分析结果明显改善，肺组织的通透性明显降低（尸〈0．01），肺组织损伤减轻。结论缺血后处理可以减轻肺缺血一再灌注损伤，主要表现为肺组织氧合的改善，尤其是减轻组织水肿和减少Ⅱ型肺泡上皮细胞及肺泡表面活性物质的破坏。Objective To investigate the effect of ischemia post-conditioning on the rat lung during the ischemia-reperfusion injury by measuring the expression levels of SP-A of the lung tissue and the variation of ar- terial blood gas. The permeability and the pathological changes of the lung tissue were observed. Methods Sixty male Sprague-Dawley rats were randomized into two model built groups with 30 animals in each group, then each model built group were randomized into five groups with 6 animals in each group： （1）sham operation group （S）. （2） I/R 45 mins group（IR45 ）. （3）I/R 120 rains group（IR120）. （4）Post-conditioning 45mins group（P45 ）, （5）post-con- ditioning 120 mins group （P120）. The expression levels of SP-A of lung tissue were detected by immuno-histo- chemical s!ain. Arterial blood gas analysis was conducted. The permeability of lung tissue was determinated （indi- cating by the leakage of ^125Ⅰ bovine serum albumin）. The pathological changes of the lung tissue were observed un- der light microscopy and electron microscopy. Results In IPO group, the level of SP-A in lung tissue increased markedly compared with I/R group. The permeability of lung tissue decreased significantly, and the pathologic le- sion of lung tissues were slight. Conclusion Post-conditioning could reduce lung I/R injury, the lung protection of IPO show by improvement of the oxygenation, relieve of lung tissue edema and inhibition of the destruction of alveolar epithelial cell type Ⅱ and alveolar surfactant especially SP-A.

关 键 词：肺 缺血后处理 再灌注损伤 肺表面活性蛋白A 血气分析 

分 类 号：R563[医药卫生—呼吸系统]