8～20周糖尿病大鼠视网膜血管内皮细胞周期阻滞  

Cell cycle arrest of retinal vascular endothelial cell of 8～20 weeks diabetic rats,

作　　者：李爱冬[1] 羊惠君[1] 韦纯义 王凡[1] 康健[2] 庞燕 

机构地区：[1]华西医科大学基础医学院解剖教研室,成都610041 [2]川北医学院

出　　处：《中华眼底病杂志》2000年第3期173-176,共4页Chinese Journal of Ocular Fundus Diseases

基　　金：四川省科委科研基金!资助 (Q0 1 2 1 )

摘　　要：目的　研究非增生性糖尿病视网膜病变血管内皮细胞的周期变化。　方法　四氧嘧啶(alloxan)诱导Wistar大鼠糖尿病模型。免疫组化光镜和电镜、Westernblotting法观察 1～ 2 0周病程糖尿病大鼠视网膜血管壁细胞细胞周期的变化。　结果　免疫光镜和电镜观察到 8～ 2 0周糖尿病大鼠视网膜血管呈cyclinD1、cyclinD3、cyclinB1、p2 1和p2 7免疫阳性反应 ,偶见内皮细胞呈cyclinE免疫阳性反应。同时可见内皮细胞细胞质减少、细胞变薄、腔面泡状结构和微绒毛增多。而此阶段周细胞和视网膜内其它类型细胞未见有超微结构变化并为免疫阴性反应。考马斯亮蓝凝胶电泳和Westernblotting亦证实视网膜内有cyclinD1、cyclinB1、p2 1和p2 7蛋白的表达。　结论　高糖导致 8～ 2 0周糖尿病大鼠视网膜血管内皮细胞进入细胞周期并阻滞于G1/S限制点 ,而周细胞没有出现相应的变化 ,本研究也提示在糖尿病早期视网膜血管内皮细胞具有拮抗高糖损伤、自我稳定的分子机制。Purpose To study changes of cell cycle of vascular endothelial cell in non-proliferative diabetic retinopathy. Methods Alloxan induced Wistar-rats were employed and immunohistochemistry,Western blotting methods were used. Results The vascular endothelial cells of retinas of 8～20 weeks diabetic rats were observe to be cyclinD1,cyclinD3,cyclinB1,p21 and p27 positive stained with light and electronmicroscopies.CyclinE immuno-stained vascular endothelial cells was observed occasionally.Meanwhile,the evidences of morphologic changes of the vascular endothelial cells were proved:less plasma,thinner cell,more bubble organelles than those of controls.But,the ultra-structures of pericytes and other type of retinal cells did not change and they also immuno-stain negative.Komas blue and Western blotting methods also proved that the vascular endothelial cells of retina of 20th week diabetic rats expressed cyclinD1,cyclinB1,p21 and p27 protein. Conclusion Glucose induced retinal vascular endothelial cells of 8～20th weeks diabetic rats enter cell cycle and were arrested at G1/S restriction point.This study also suggested that retinal vascular endothelial cells may possess the ability to resist glucose damage and mechanism of selfstability during very early stage of diabetes.

关 键 词：细胞周期 糖尿病视网膜病变 血管内皮细胞 

分 类 号：R587.2[医药卫生—内分泌] R774.130.2[医药卫生—内科学]