Fe^(3+)-H_2O_2-钙黄绿素-邻菲啰啉化学发光体系测定左旋多巴分析方法研究  

Analysis method for determination of levodopa using Fe^(3+)-H_2O_2-calcein-phenanthroline morpholine chemiluminescence system

作　　者：邱霞琳[1] 蔡卓[1] 何晓良[1] 岳伟超[1] 蒋翠文[1] 

机构地区：[1]广西大学化学化工学院,广西南宁530004

出　　处：《化学研究与应用》2013年第5期701-705,共5页Chemical Research and Application

基　　金：广西自然科学基金项目(2010GXNSFA013001)资助;广西大学实验设备处项目(20120301)资助

摘　　要：提出了Fe3+-H2O2-钙黄绿素化学发光体系并用于左旋多巴的测定。在酸性介质中,Fe3+-H2O2-钙黄绿素-邻菲啰啉体系能产生明显的化学发光现象。加入左旋多巴后,对此化学发光强度具有显著的抑制作用,降低程度与体系中左旋多巴浓度呈良好的化学计量关系。据此,结合流动注射技术,建立了流动注射化学发光测定左旋多巴的方法。研究了影响化学发光强度的因素,并探讨了可能发生的机理。化学发光信号的降低值(ΔI)与左旋多巴浓度在2×10-8mol.L-1～2×10-6mol.L-1的范围内呈良好的线性关系,检出限为1.2×10-8mol.L-1。相对标准偏差为1.1%,该法用于片剂中左旋多巴含量的测定,结果令人满意。A novel chemiluminescence （CL） system was established for the determination of Levodopa （L-dopa） in pharmaceutical preparations. In acid medium, Fe3＊-H202-calcein-I, 10-Phenanthroline system can produce a significant chemiluminescence. It was showed that a comparatively inhibitory effect in CL signal was observed when L-dopa was added to the reaction system. The experi- mental parameters that affected the CL intensity were investgated. The decrease extent of the CL intensity was of a good stoichiomet- rical relationship with L-dopa concentration. Based on this, a new method for determination of L-dope using flow injection chemilu- minescence technique was developed. Various factors influencing the CL system were investigated and the possible mechanism was also discussed. Under the optimal conditions, the linear range for L-dope concentration was 2.0×10^-8 - 2. 0 ×10^-6 mol· L-1 with a detection limit of 1.2×10^-8 mol·L-1 ,the relative standard deviation was 1. 1%for 2. 0×10^-7 mol· L-1 L-dopa（n= 11 ）. This method was successfully used in the determination of puerarin in injection, The method was applied to the determination of L-dope in injection with satisfactory results.

关 键 词：流动注射 化学发光 钙黄绿素 左旋多巴 

分 类 号：O657.3[理学—分析化学]