构树叶中的细胞毒成分(英文)  

Cytotoxic constituents from the leaves of Broussonetia papyrifera

作　　者：冉小库[1] 王晓彤[1] 刘培培[1] 迟玉新[1] 王博佳[1] 窦德强[1] 康廷国[1] 熊伟 

机构地区：[1]辽宁中医药大学药学院,大连116600 [2]大连快活林生物技术有限公司,大连116600

出　　处：《中国天然药物》2013年第3期269-273,共5页

摘　　要：目的:研究构树叶的化学成分。方法:采用D101型大孔吸附树脂、硅胶、ODS和半制备型高效液相色谱等分离方法对构树叶提取物分离纯化,通过1D,2DNMR技术确定其结构,并采用MTT法对分得化合物进行细胞毒活性测定,同时比色法和采用高效液相色谱法建立了对构树叶中总黄酮和cosmosiin的含量测定方法。结果:分离鉴定了6个化合物,它们的结构鉴定为:(+)-pinoresinol-4′-O-β-D-glucopyransyl-4″-O-β-D-apiofuranoside(1),cosmosiin(2),luteolin-7-O-β-D-glucopyranoside(3),liriodendrin(4),3,5,4′-trihydroxy-bibenzyl-3-O-β-D-glucoside(5),apigenin-6-C-β-D-glycopyranside(6)。结论:化合物1为一个新的木脂素,化合物5,6为首次从该属植物中分离,化合物1,4,6对HepG-2细胞株有不同程度的抑制活性,而化合物2,3,5对HepG-2细胞株没有活性;根据含量测定结果得知,确定构树叶的最佳采收时间为9月份。AIM： To investigate the chemical constituents from the leaves of Broussonetia papyrifera. METHODS： The chemical constituents were isolated and purified by macroporous adsorptive resin D101, silica gel, and ODS column chromatography and prepa- rative HPLC. Their structures were elucidated on the basis of 1D and 2D NMR analyses. In addition, their cytotoxic activity against human hepatoma carcinoma cells （HepG-2） were evaluated by the MTT method Furthermore, RP-HPLC and colorimetric methods were used for the analysis of cosmosiin and total flavonoids. RESULTS： A new lignan, together with five known compounds were obtained, and their structures were characterized as （＋）-pinoresinol-4＇-O-fl-D-glucopyranosyl-4＂-O-fl-D-apiofuranoside （1）, cosmosiin （2）, luteolin-7-O-fl-D-glucopyranoside （3）, liriodendrin （4）, 3, 5, 4＇-trihydroxy-bibenzyl-3-O-fl-D-glucoside （5）, and apigenin-6-C-fl-D- glucopyranside （6）. Furthermore, RP-HPLC and colorimetric methods were established for the analysis of cosmosiin and total flavon- oids. CONCLUSION： Compound 1 was a new lignan, and compounds 5 and 6 were isolated for the first time from the title plant. Compounds 1, 4 and 6 showed definite activities against HepG-2, while the other compounds didn＇t show inhibitory effects. The opti- mal harvest time of B. papyrifera （L.） Vent. is September.

关 键 词：枸树 桑科 木脂素 HPLC 细胞毒活性 

分 类 号：R284.1[医药卫生—中药学]