小鼠长期口服阿苯达唑后停药对受损肝组织和功能的影响  

The influence of withdrawing long-term oral administration of albendazole on impaired liver tissue and function in mice

作　　者：郑琪[1] 刘丛珊[1] 姜斌[1] 徐莉莉[1] 张皓冰[1] 

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所卫生部寄生虫病原与媒介生物学重点实验室世界卫生组织疟疾、血吸虫病和丝虫病合作中心,上海200025

出　　处：《国际医学寄生虫病杂志》2013年第3期135-139,共5页International JOurnal of Medical Parasitic Diseases

摘　　要：目的观察小鼠长期t3服阿苯达唑后，停药对受损肝组织和肝功能恢复的可能性。方法雌性昆明系小鼠110只．其中90只作为实验组，每鼠每天灌服剂量为136．3mg／kg的阿苯达唑混悬液。另外20只作为对照组，每鼠每天灌服生理盐水10ml／kg。上述2组鼠于服药12周，实验组和对照组各取10只小鼠剖杀．从眼内眦静脉取血作肝功能测定．并从各组的10只小鼠中随机取5只的肝左叶组织，作肝细胞透射电镜观察。与此同时．将实验组的剩余80只小鼠均分为继服药组和停药组，继服药组每天灌服上述剂量的阿苯达唑．继服4周，而停药组和对照组余留的10只小鼠则灌服上述剂量的生理盐水4周。继服药组在继服阿苯达唑1、2、3、4周，或停药组于停药1、2、3、4周后各取10只小鼠剖杀，对照组的10只小鼠则于灌服生理盐水16周后剖杀．并按上述方法取小鼠的眼内眦静脉和肝脏作肝功能测定和肝细胞电镜观察。结果小鼠继服药1-4周组血清丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、碱性磷酸酶（ALP）活力逐周下降．小鼠灌服阿苯达唑12周．停药1周后各组的上述3种酶活力亦明显下降。其中1周停药组ALT值（39．6±6．2）明显低于同期继服药组（45．6±8．5）．（Z=1．82，P〈0．05），1周和2周停药组的AST值137．8±11．9、120．6±9．3显著低于同期继服药组162．4±25．7、141．0±15．1（ZAST1=1．92，ZAST2=1．75，P均〈0．05）；1周和2周停药组的ALP值120．6±15．2、104．6-4-15．7明显低于同期继服药组142．6±8．4、125．3±11．6（ZALP1=1．86，ZAST2=1．55，P均〈0．05）。继服药1-4周组肝细胞病理变化程度与服药12周组相比较．呈逐周减轻．停药1～4周组各组小鼠病理变化的减轻较继服药组迅速和明显．停药1周组和2周组小鼠肝细胞透射电镜病理变化评分由23．4±6．3．分别降至6Objective To investigate the possibility of liver functional recovery by withdrawing long term o- ral administration of abendazole in mice. Methods One hundred and ten female mice were divided into two groups. Ninety of them were treated with albendazole at the dosage of 136.3 rag/ （kg.d） by stomach garage. The other 20 mice as control group were treated with physiological saline. Ten mice were randomly picked up from each group after treatment for 12 weeks, mice were executed and blood collected from lateral canthus of eyes for liver function detection. Five mice were picked up from each 10 mice to collect liver tissues for pathology observation under trans- mission electron microscope. Meanwhile, the 80 mice in treated group were equally divided into continuously treating group and withdraw group to treat for another 4 weeks. Ten mice were randomly picked up from continuously treating group and withdrawing group after treatment for 1, 2, 3, 4 weeks, as well as 10 mice in control group in 16 weeks. The blood and liver tissue were collected for same detection as described above. Results The values of ALT, AST and ALP in continuously treating group decreased by weeks. The values of ALT, AST and ALP also dramatically declined after withdrawing treatment for more than 1 week. The values of ALT in withdrawaing 1 week group （39.6 ± 6.2） were significantly lower than those in continuously treating group （45.6 ± 8.5） （Z=1.82, P〈0.05）. The values of AST in withdrawaing 1 week and 2 week group （137.8 ± 11.9, 120.6 ± 9.3） were significantly lower than those in continuously treating group （162.4 ± 25.7, 141.0 ± 15.1） （ZAST1=1.92, ZAST2=1.75, P〈0.05）. The values of ALP in withdrawaing 1 week and 2 week group （120.6 ± 15.2,104.6 ±15.7） were significantly lower than those in continuously treating group （142.6 ± 8.4, 125.3 ± 11.6） （ZALP1=1.86, ZALP2=1.55, P〈0.05）. Compared to treatment 12 week group, the pathological changes in continuously treating group and withdrawaing

关 键 词：棘球蚴病 阿苯达唑 不良反应 肝损伤 

分 类 号：R392-33[医药卫生—免疫学]