EB病毒DNA监测对传染性单核细胞增多症诊断的重要性研究  

Detection of EBV-DNA in blood of patients with infectious mononucleosis and its clinical significance

作　　者：吴雪梅[1] 

机构地区：[1]遵义市第一人民医院儿科,贵州遵义563000

出　　处：《中华医院感染学杂志》2013年第10期2280-2281,2286,共3页Chinese Journal of Nosocomiology

摘　　要：目的探讨EB病毒DNA监测在传染性单核细胞增多症(IM)中的临床应用价值,以指导病情的评估。方法收集儿科2011年6月-2012年8月治疗的IM患者66例,入院2d及7d荧光定量PCR检测EB病毒DNA的表达。结果入院2d检测EBV-DNA阳性率为89.4%、7d检测阳性率为92.4%,两者差异无统计学意义;不同EBV-DNA拷贝数的患儿肝脾肿大程度差异有统计学意义(P<0.05);临床热程在1×103～1×105、1×105～1×107和>1×107组分别为(6.12±3.22)d、(7.43±4.65)d和(9.16±5.24)d,各组之间差异有统计学意义(P<0.05);不同EBV-DNA拷贝数的患儿谷丙转氨酶、谷草转氨酶、肌酸激酶、肌酸激酶同工酶差异也有统计学意义(P<0.05)。结论荧光定量PCR检测EBV-DNA可以用于IM的早期诊断以及患儿病情的评估。OBJECTIVE To investigate the clinical significance of EBV-DNA in patients with infectious mononucleosis （IM） so as to guide the evaluation of the condition. METHODS In the hospital from Jun 2011 to Aug 2012, totally 66 IM patients were collected. After the admission for 2d and 7d, the fluorescence quantitative PCR was employed to detect the EB virus DNA expression two days and seven days after the admission. RESULTS The positive rate of EBV-DNA was 89.4% 2 days after the admission and 92.4% 7 days after the admission, the difference between the two was not statistically significant. The hepatosplenomegaly extent had a significant difference among different EBV-DNA copy number in children with IM（P〈0.05）. The clinical thermal process in 1 ×10^3-1 × 10^5 , 1×10^-1×10^7, and 〉1×10^7 group were （6.12±3.22） days, （7.43±4.65） days, and （9.16± 5.24 ） days, respectively. There was significant difference among the different groups（P〈0.05）. The ALT, AST, CK, and CK-MB also had a significant difference among different EBV-DNA copy number in the children with IM（P〈 0.05）. CONCLUSION Quantitative PCR detection of EBV-DNA can be used for the early diagnosis of IM as well as for the assessment of process of the disease.

关 键 词：传染性单核细胞增多症 EB病毒DNA 荧光定量PCR 

分 类 号：R373[医药卫生—病原生物学]