斜带石斑鱼(Epinephelus coioides)饲料中铜的适宜添加量研究  

DIETARY COPPER REQUIREMENT OF JUVENILE GROUPER EPINEPHELUS COIOIDES

作　　者：叶超霞[1,2] 刘永坚[2] 田丽霞[2] 阳会军[2] 王安利[1] 周立斌[3] 

机构地区：[1]华南师范大学生命科学学院广东省水产健康安全养殖重点实验室,广州510631 [2]中山大学水生经济动物研究所,广州510275 [3]惠州学院生命科学系,惠州516007

出　　处：《海洋与湖沼》2013年第2期461-466,共6页Oceanologia Et Limnologia Sinica

基　　金：国家科技支撑计划项目,2007BAD29B04号;国家自然科学基金项目,31100296号;广东省重点实验室建设项目,2009A060800019号;广东省自然科学基金项目,SZ2011010005079号;惠州市科技计划项目,2011B040010003号

摘　　要：以初始体重为(13.0±0.3)g的斜带石斑鱼(Epinephelus coioides)为实验对象,用铜添加量为0、1、3、5、10和20mg/kg的实验饲料投喂斜带石斑鱼8周,研究斜带石斑鱼的铜需求量。结果表明,饲料中添加0—20mg/kg铜对石斑鱼生长、饲料效率、存活率、形态学指标和红细胞比容没有显著性影响。随饲料铜水平的上升,斜带石斑鱼全鱼和肝脏的铜含量呈先上升后平稳的趋势,当饲料铜添加量达到或超过3mg/kg时,石斑鱼全鱼和肝脏的铜含量趋于平稳。折线模型表明,饲料中铜总含量为5—5.5mg/kg时,石斑鱼全鱼和肝脏的铜含量能够达到饱和。当饲料中铜总含量≥6.00mg/kg时,全鱼的铁、锰、锌含量显著下降。因此,根据全鱼和肝脏铜含量的折线模型分析,考虑到饲料铜对全鱼铁、锰、锌含量的影响,作者认为当基础饲料铜含量为1.50mg/kg时,饲料中的最适铜添加量为3mg/kg,饲料中铜总量为5—5.5mg/kg左右。This study was conducted to investigate the copper requirement of juvenile grouper, Epinephelus coioides. Six diets were prepared by supplementing a casein-gelatin-based diet with 0, 1, 3, 5, 10 and 20mg/kg copper from CuSO4＇5H2O. Grouper with an initial weight of （13.0±0.3）g were fed to satiation for 8 weeks. Growth performance, feed utilization, morphological indicators and hematocrit value of juvenile grouper were not significantly affected by dietary copper supplements ranging from 0 to 20mg/kg. Copper concentration of whole body and liver responded linearly to die- tary copper supplement up to 3mg/kg and then reached a plateau. Broken-line analysis indicated that dietary copper of 5-- 5.5mg/kg satisfied whole body and liver copper storage. Whole body Fe, Mn and Zn were significantly lower when dietary total copper≥ 6mg/kg. Liver Fe, Mn and Zn were not significantly affected by dietary copper supplement ranging from 0 to 20mg/kg. Concerning broken-line analysis of whole body and liver Cu, and the interaction effect of Cu supplement on whole body Fe, Mn and Zn concentration, we estimate that copper supplement of 3mg/kg might be optimum to the basal diet containing 1.50mg/kg Cu, providing dietary total copper of 5--5.5mg/kg.

关 键 词：斜带石斑鱼 铜 矿物质 

分 类 号：S963[农业科学—水产养殖]