HPLC法测定清肺止咳颗粒中木兰脂素、秦皮甲素、秦皮乙素的含量  

Determination of magnolin,aesculin and aesculetin in Qingfeizhike Particles by HPLC

作　　者：胡海聪[1] 于珊珊[1] 张晓静[1] 张俊义[1] 孙毅坤[1] 

机构地区：[1]北京中医药大学,北京100102

出　　处：《药物分析杂志》2013年第5期866-869,873,共5页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:测定清肺止咳颗粒中木兰脂素、秦皮甲素、秦皮乙素的含量。方法:采用HPLC法,使用十八烷基硅烷键合硅胶色谱柱DiamonsiL C18(250 mm×4.6 mm,5μm)。测定木兰脂素的流动相为乙腈-水(40∶60),流速1 mL.min-1,检测波长为278 nm;测定秦皮甲素、秦皮乙素的流动相为乙腈-0.1%磷酸溶液(8∶92),流速1.0 mL.min-1,检测波长为334 nm。结果:木兰脂素、秦皮甲素、秦皮乙素进样量分别在0.22～1.76μg(r=0.9998)、0.102～0.306μg(r=0.9997)、0.23～0.69μg(r=0.9997)范围内线性关系良好;平均回收率为99.9%、97.7%、100.0%,RSD为1.4%、2.0%、1.8%。结论:所建立的分析方法可作为清肺止咳颗粒的质控标准。Objective： To determine the content of magnolin, aesculin, aesculetin in Qingfeizhike Particles by HPLC. Methods：An HPLC method with a Diamonsil C lS column（250 mm×4.6 mm,5μm） was used. For magnol-in,the solution of acetonitrile - water （40： 60） was used as the mobile phase at a flow rate of 1.0 mL·min^-1 and a detection wavelength of 278 nm ; for aesculin and aesculetin, acetonitrile -0. 1% phosphoric acid （ 8 ： 92 ） wasused as the mobile phase at a flow rate of 1.0 mL·min^-1 and a detection wavelength of 334 nm. Results：The linear relationships of magnolin, aesculin and aesculetin were good within the ranges of 0.22～1.76μg（r=0.9998）、0.102～0.306μg（r=0.9997） and 0.23～0.69μg（r=0.9997） , respectively, and the average recovery rates were 99.9% ,97.7% and 100. 0%, respectively, with RSD of 1.4%, 2.0% and 1.8%. Conclusion： The establishedmethod can be used for the quality control standard of Qingfeizhike Particles.

关 键 词：清肺止咳颗粒 木兰脂素 秦皮甲素 秦皮乙素 高效液相色谱法 中药复方制剂 质量控制标准 

分 类 号：R917[医药卫生—药物分析学]