炭样小单孢菌JXNU-1产抗生素对G^-的体外抗菌作用及其机理的初步研究  

Studies on the Antimicrobial Activity and Its Mechanism of an Antibiotic,from Micromonospora carbonacea JXNU-1,Against Gram-Negative Bacteria in Vitro

作　　者：彭伟梦[1] 黄运红[1] 李瑾[1] 高兴强[1] 龙中儿[1] 

机构地区：[1]江西师范大学生命科学学院,江西南昌330022

出　　处：《江西师范大学学报（自然科学版）》2013年第2期162-165,共4页Journal of Jiangxi Normal University(Natural Science Edition)

基　　金：国家自然科学基金(31160029);江西省自然科学基金(20122BAB204008)资助项目

摘　　要：基于土壤中分离到的一株具广谱抗菌活性的炭样小单孢菌JXNU-1,研究了JXNU-1所产抗生素对G-的体外抗菌作用.研究结果表明:炭样小单孢菌JXNU-1所产抗生素对产气肠杆菌、伤寒沙门氏菌、铜绿假单孢菌、福氏志贺氏菌等常见G-细菌均有抗菌作用,其最低杀菌浓度一般是最低抑菌浓度的8～16倍.杀菌曲线表明其对G-的杀菌作用非常快速,8 h的杀菌率已超过90%.进一步的研究表明抗生素抗G-的作用机理在于抑制细菌蛋白质的合成,进而抑制细胞的分裂.Based on the strain of Micromonospora carbonacea JXNU-1 with board-spectrum antimicrobial activity, isolated from a soil sample, the antibacterial activity of its antibiotics against gram-negative bacteria in vitro was studied. The results indicated that the antibiotics had antimicrobial activity against common gram-negative bacteria, such as Enterobacter aerogenes, Salmonella typhi, Pseudomonas aeruginosa, Shigella flexneri and so on. The killing curve proved that the bactericidal action was very rapidly, the sterilizing rate over 90% when germs were treated with antibiotics for 8 hours,and the MBC usually 8 to 16 times of the MIC. Further more,studies have shown that the mechanism of the antibiotics against gram-negeative bacteria is to inhibit bacterial protein synthesis, and thereby inhibiting cell division.

关 键 词：炭样小单孢菌 抗生素 革兰氏阴性菌 抗菌作用 

分 类 号：Q939.9[生物学—微生物学]