安徽水稻黑条矮缩病毒基因组片段S10的cDNA克隆及其序列分析  

cDNA Clone and Sequence Analysis of Segment 10 of Rice Black-Streaked Dwarf Fijivirus in An hui Province

作　　者：张海珊[1] 顾江涛[1] 严丹侃[1] 周桃棣 夏必文 刘轩武 章东方[1] 

机构地区：[1]安徽省农业科学院植物保护与农产品质量安全研究所,安徽合肥230031 [2]太湖县植保站,安徽太湖246400 [3]巢湖市居巢区植保站,安徽巢湖238000 [4]全椒县植保站,安徽全椒239500

出　　处：《安徽农学通报》2013年第9期31-31,73,共2页Anhui Agricultural Science Bulletin

基　　金：安徽省种子工程项目(09B0901)

摘　　要：从采自安徽省肥西县发病的水稻材料中提取水稻黑条矮缩病毒dsRNA,利用RT-PCR获得了病毒基因组片段S10的cDNA克隆,并测定其全序列。结果表明:S10全长1801bp,含有一个ORF;核苷酸与氨基酸序列同源性比较表明,其与浙江的RBSDV(AY050488)最接近,分别为99.2%和99.6%;与日本的RBSDV(D00606)分别为95.1%和97.5%。The infected rice plants sample of rice black streaked dwarf virus （RBSDV） was collected from Feixi county of Anhui province. Virus genome segment 10（SIO） cDNA clone was obtained by RT-PCR. Sequence analysis results showed that the full length of SIO is 1801bp and has one ORF. The similarity of nueleotide and predicted protein were 99.2% and 99.6% with RBSDV（AY050488） from Zhejiang,95.1% and 97.5% with RBSDV （DOO606）from Japan.

关 键 词：水稻黑条矮缩病毒 CDNA克隆 S10片段 序列分析 

分 类 号：S435.11[农业科学—农业昆虫与害虫防治]