菊三七根中化合物seneciphyllic acid和senecinic acid的含量测定  

Content Determination of Seneciphyllic Acid and Senecinic Acid in Roots of Gynura Japonica

作　　者：刘芳[1] 郭志勇[1] 程凡[1] 周海峰[1] 邹坤[1] 邓张双[1] 

机构地区：[1]三峡大学化学与生命科学学院天然产物研究与利用湖北省重点实验室,湖北宜昌443002

出　　处：《三峡大学学报（自然科学版）》2013年第2期97-99,共3页Journal of China Three Gorges University：Natural Sciences

基　　金：国家自然科学基金(31070313;21272136);湖北省自然科学基金(2011CDB177);三峡大学人才科研启动基金(KJ2011B003)

摘　　要：目的:采用HPLC法测定菊三七根部seneciphyllic acid和senecinic acid的含量.方法:建立HPLC法测定菊三七根部seneciphyllic acid和senecinic acid的含量.色谱柱为Acclaim C18色谱柱(4.6mm×250mm,5μm),流动相为甲醇-0.1%甲酸水(40:60,v.v-1),流速为1.0mL.min-1,柱温为30℃,紫外检测波长为220nm.线性范围:Seneciphyllic acid为0.1～0.8mg.mL-1(相关系数r=0.999 7);senecinic acid为0.1～1.5mg.mL-1(相关系数r=0.999 8).结果:测得菊三七根部seneciphyllic acid和senecinic acid的含量分别为2.961 3mg.g-1、0.954 2mg.g-1;RSD分别为1.34%、1.28%;平均回收率为98.84%、99.16%,RSD为1.94%、1.51%.结论:该方法快速、简便、准确度高,可为开发利用菊三七提供研究基础.The contents of seneciphyllic acid and senecinic acid in root of Gynura Japonica were determined by HPLC and the method of HPLC determination was established . The analysis was operated on a Acclaim C18 column（4.6 minx 250 mm,5μm）at 30℃ with mobile phase contained methanol-0. 1% formic acid water （40 ： 60,v·v-1）at a flow rate of 1 mL·min-1 followed by UV detection at 220 nm. The calibration curve of seneciphyllie acid was well linear over the concentration rang from 0.1 to 0.8 mg·mL-1 （r=0. 9997）;; the calibration curve of senecinic acid was well linear over the concentration rang from 0.1 to 1.5 mg·mL-1 （r= 0. 9998）. The contents of seneciphyllic acid and senecinic acid in root of Gynura Japonica are 2. 9613mg·g-1,0. 9542mg·g-1, respectively. The average recovery of seneciphyllic acid and senecinie acid are 98.84%, 99.16% with a RSD of 1.94%, 1.51%. This method is fast, simple and high accuracy and will provide tech-nology for the development and utilization of Gynura Japonica.

关 键 词：Seneciphyllic ACID senecinic ACID 菊三七 含量测定 HPLC 

分 类 号：R284[医药卫生—中药学] Q586[医药卫生—中医学]