三维试验检测耐头孢他啶铜绿假单胞菌产β-内酰胺酶  

Three-dimensional method applied to detect beta-lactamases in ceftazidime-resistant P.Aeruginosa

作　　者：陈红 

机构地区：[1]湖南省益阳市第三人民医院检验科,413000

出　　处：《检验医学与临床》2013年第10期1210-1211,共2页Laboratory Medicine and Clinic

摘　　要：目的了解耐头孢他啶铜绿假单胞菌产β-内酰胺酶的现状。方法采用K-B法进行药敏试验,筛选耐头孢他啶的铜绿假单胞菌,采用改良三维试验检测各种β-内酰胺酶。结果 65株临床分离耐头孢他啶铜绿假单胞菌中产超广谱β-内酰胺酶(ESBLs)30株(46.2%),AmpC酶4株(6.2%),同时产ESBLs和AmpC酶10株(15.4%),同时产金属酶和产ESBLs 15株(23.1%),同时产AmpC酶和金属酶2株(3.1%)。结论益阳市第三人民医院铜绿假单胞以产ESBLs为主,其次为AmpC酶和金属酶。Objective To analyze beta-lactamase in ceftazdimine-resistant P. Aeruginosa. Methods The strains of ceftazidime -resistant P. Aeruginosa were selected with K-B susceptibility method. Three-dimensional method was used to differentiate and analyze the various heta-laetamases. Results In 65 strains ceftazidime-resistant P. Aerugino- sa, 40 strains （46.2% ） could produce expended spectrum beta-lactamases （ESBLs） ,4 strains （6.2%） could produce AmpC,10 strains （15.4%） could produce both ESBLs and AmpC, 15 strains （23.1%） could produce both metallo- beta-lactamases and ESBLs, 2 strains （3.1%） could produce both AmpC and metallo-beta-lactamases. Conclusion The ceftazidime-resitant P. Aeruginosa strain producing ESBLs might be priority, followed by AmpC enzyme and metallo-beta-lactamases.

关 键 词：铜绿假单胞菌 Β-内酰胺酶 三维实验 耐药 头孢他啶 

分 类 号：R978.1[医药卫生—药品]