盐胁迫对2种苹果属植物愈伤组织及组培苗生长和有机渗透调节物质累积的影响  

Effects of Salinity Stress on Growth and Organic Osmolytes Accumulation of Callus and Tissue Culture Seedlings of Two Malus

作　　者：王锴[1] 张立新[1] 高梅[1] 赵永桂[1] 吕丽霞[2] 张林森[3] 李丙智[3] 韩明玉[3] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨凌712100 [2]西北农林科技大学林学院,陕西杨凌712100 [3]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《西北农业学报》2013年第2期112-118,共7页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家苹果产业技术体系(CARS-28);陕西省农业科技创新项目(2011NXC01-18);中国科学院"西部之光"人才支持项目(2060299-14);陕西省重大科学技术难题攻关项目(2011KTZB02-02-05)

摘　　要：以苹果属植物八棱海棠(Malus robusta Rehd)和富士(Malus pumila cv.Fuji)为材料,在含0和150mmol/L NaCl的MS培养基中培养15d,研究盐胁迫对2种苹果属植物愈伤组织及组培苗生长和有机渗透调节物质累积的影响,明确其耐盐适应性机制。结果表明,盐胁迫下八棱海棠和富士愈伤组织及组培苗的可溶性蛋白、可溶性糖和脯氨酸含量升高,相对生长速率(RGR)降低,且愈伤组织生长受影响更大。其中八棱海棠愈伤组织可溶性蛋白、可溶性糖和脯氨酸含量分别比对照上升380.6%、25.5%和130.2%,组培苗分别比对照上升7.1%、9.0%和449.0%;富士愈伤组织可溶性蛋白、可溶性糖和脯氨酸含量分别比对照上升30.1%、27.5%和385.0%,组培苗分别比对照上升22.0%、90.0%和412.4%。以上结果表明,2种苹果属植物愈伤组织和组培苗均可通过诱导渗透调节物质的累积来适应盐胁迫,其中八棱海棠愈伤组织以可溶性蛋白累积最显著,而富士愈伤组织以及2种苹果属植物组培苗均以脯氨酸累积最显著,其愈伤组织生长对盐胁迫比组培苗更敏感。The two Malus i. e. Malus robusta Rehd and Malus pumila cv. Fuji were cultured in Mu- rashige and Skoog （MS） medium contained 0 mmol/L and 150 mmol/L NaC1 for 15 days to study the effects of salinity stress on growth and organic osmolytes accumulation in callus and tissue culture seedlings. The salinity stress stimulated by NaC1 in MS medium caused an increase of accumulation of soluble protein, soluble sugars and proline while a decrease o~[ relative growth rate of both callus and tissue culture seedlings of two Malus. The effects of salinity on growth of callus were greater than tis- sue culture seedling. Among them, the contents of soluble protein, soluble sugars and proline en- hanced by 380.6G, 25.5% and 130.2% in Rehd callus, and by 7.1%, 9.0% and 449.0% in Rehd tissue culture seedlings. As for Fuji, soluble protein, soluble sugars and proline contents increased by 30.1%, 27.% and 385.0% in callus, and by 22.0%, 90.0% and 412.4% in tissue culture seedlings. All the above results indicated that both callus and tissue culture seedlings of two Malus can be adaptive to salinity stress by accumulation of organic osmolytes. The accumulation of soluble protein was the most noted in Rehd callus, while that of proline was the greatest in Rehd tissue culture seed- lings as well as both callus and tissue culture seedlings of Fuji. The growth of two Malus callus was more sensitive than tissue culture seedlings in responses to salt stress.

关 键 词：盐胁迫 苹果 愈伤组织 组培苗 渗透物质 

分 类 号：S661.1[农业科学—果树学]